| 桑黄化型萎缩病病原体16S rRNA基因的序列分析 |
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| 引用本文： | 夏志松,難波成任.桑黄化型萎缩病病原体16S rRNA基因的序列分析[J].蚕业科学,2004,30(2):204-206. |
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| 作者姓名： | 夏志松 難波成任 |
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| 作者单位： | 中国农业科学院蚕业研究所,镇江,212018;日本东京大学农学部,东京,1138657 |
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| 基金项目： | 国家留学基金高级访问学者研究项目 |
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| 摘 要： | 用PCR法克隆了中国桑黄化型萎缩病病原植原体的 16SrRNA基因 ,并进行了序列分析。结果表明 :克隆的基因大小为 1372bp ,与日本桑萎缩病病原植原体 16SrRNA的同源性高达 99 85 % ,只存在个别碱基的突变。Blastj检索结果显示与中国桑黄化型萎缩病病原体 16SrRNA的基因同源性高达 99%的有来源于玉米、洋葱、土豆等5 0多种其它植物的植原体 16SrRNA基因 ,表明植物萎缩病病原体 16SrRNA基因具高度保守性。
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| 关 键 词： | 桑树 桑黄化型萎缩病 植原体 16S rRNA基因 序列分析 |
| 文章编号： | 0257-4799(2004)02-0204-03 |
| 修稿时间： | 2004年1月2日 |
Cloning and Sequencing of Phytoplasma 16S rRNA Gene From Infected Mulberry in China |
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| XIA Zhisong.Cloning and Sequencing of Phytoplasma 16S rRNA Gene From Infected Mulberry in China[J].Acta Sericologica Sinica,2004,30(2):204-206. |
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| Authors: | XIA Zhisong |
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| Abstract: | The 16S rRNA gene of phytoplasma from infected mulberry in China was cloned by PCR method and sequenced.It showed that the full length of geme was 1 372 bps and had 99.85% homology with the counterpart in Japan,which only existed several bases mutation.Moreover,through blasting in NCBI,there are more than 50 phytoplasma 16S rRNA genes which derived from infected plants such as maize,onion,potato etc and had above 99% homology with 16S rRNA gene of phytoplasma from infected mulberry in China.It shows that the phytoplasma 16S rRNA gene is a high conserve one. |
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| Keywords: | MulberryMulberry dwarf diseasePhytoplasma16S rRNA geneSquencing analysis |
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