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Recovery of Moraxella ovis from the bovine respiratory tract and differentiation of Moraxella species by tDNA-intergenic spacer PCR |
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| Authors: | Catry Boudewijn Boyen Filip Baele Margo Dewulf Jeroen de Kruif Aart Vaneechoutte Mario Haesebrouck Freddy Decostere Annemie |
| Institution: | aDepartment of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium bDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium cDepartment of Clinical Chemistry, Microbiology & Immunology, University Hospital Ghent, De Pintelaan 185, 9000 Ghent, Belgium |
| Abstract: | The purpose of the present study was to identify Moraxella (M.)—like organisms recovered from calves suffering from respiratory disease down to species level by means of tDNA-intergenic spacer length polymorphism analysis (tDNA-PCR), and to perform antimicrobial susceptibility testing of these isolates using an agar dilution technique. A total of 16 isolates originating from 12 unrelated occasions were identified as Moraxella ovis, and tDNA fingerprinting showed clear delineation from other Moraxella species. The minimal inhibitory concentrations (in μg/mL) for 90% of the investigated isolates were ≤0.03 for ampicillin; 0.25 for ceftiofur; 0.5 for oxytetracycline; 8 for gentamicin; 64 for spectinomycin; 0.5/9.5 for the combination trimethoprim-sulfonamides; 4 for erythromycin; 8 for tilmicosin; 1 for florfenicol and 0.125 for enrofloxacin. |
| Keywords: | Moraxella ovis Bovine respiratory disease Antimicrobial susceptibilities MIC |
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