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石河子地区隐性乳房炎奶牛源大肠杆菌的分离鉴定及耐药性分析
引用本文:许丹丹,许追,齐向涛,欧都,杨莉,齐亚银,剡根强.石河子地区隐性乳房炎奶牛源大肠杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2015,42(3):701-707.
作者姓名:许丹丹  许追  齐向涛  欧都  杨莉  齐亚银  剡根强
作者单位:石河子大学动物科技学院, 石河子 832000
基金项目:国家自然基金(31260610);兵团重大科技项目(2013AA003-3);石河子大学高层次人才科研启动专项(RCZX201106)
摘    要:为了解引起石河子地区规模化奶牛场隐性乳房炎奶牛源大肠杆菌的流行情况及其耐药性,本试验采用兰州乳房炎检测板(Lanzhou mastitis test,LMT)对石河子地区11个规模化奶牛场进行了检测,同时对无菌采集的检测为阳性的乳样进行了大肠杆菌分离鉴定及耐药性分析.结果显示,石河子地区11个规模化奶牛场的隐性乳房炎感染阳性率为44.56%(313/1950);对313份无菌采集的乳样进行细菌分离鉴定,结果共分离出病原菌108株,其中大肠杆分离率为14.81%(16/108);药敏试验结果显示16株大肠杆菌对氧氟沙星、头孢唑啉、克林霉素高度敏感,对庆大霉素、氨苄西林、链霉素中度敏感,对阿莫西林、四环素有耐药性.本试验为该地区大肠杆菌源性隐性乳房炎的防制提供科学依据.

关 键 词:奶牛隐性乳房炎  大肠杆菌  药敏试验  
收稿时间:2014-09-10

Isolation,Identification and Drug Resistance Analysis of Escherichia coli in Subclinical Mastitis Cow in Shihezi Region
XU Dan-dan;XU Zhui;QI Xiang-tao;OU Du;YANG Li;QI Ya-yin;YAN Gen-qiang.Isolation,Identification and Drug Resistance Analysis of Escherichia coli in Subclinical Mastitis Cow in Shihezi Region[J].China Animal Husbandry & Veterinary Medicine,2015,42(3):701-707.
Authors:XU Dan-dan;XU Zhui;QI Xiang-tao;OU Du;YANG Li;QI Ya-yin;YAN Gen-qiang
Institution:College of Animal Science and Technology, Shihezi University, Shihezi 832000, China
Abstract:In order to find out the epidemic situation and drug resistance of Escherichia coli in subclinical mastitis cow from large-scale dairy farms in Shihezi region,the method of Lanzhou mastitis test (LMT) was adopted to test 11 large-scale dairy farms around Shihezi.Meanwhile,we separated and identified Escherichia coli from positive milk samples collected under aseptic conditions.Also,drug resistance of these isolates were tested.The results showed that the positive rate of recessive mastitis in large-scale dairy farms in Shihezi area was 44.56%.After separation and identification of 313 milk samples collected under aseptic conditions,108 strains of pathogenic bacteria were isolated,and the separation rate of Escherichia coli was 14.81%.The results of drug sensitivity test showed that 16 stains of Escherichia coli were highly sensitive to ofloxacin,cefazolin and clindamycin,moderately sensitive to gentamicin,ampicillin and streptomycin,drug-resistant to amoxicillin and tetracycline.This study provided scientific basis for prevention and treatment of recessive mastitis caused by Escherichia coli.
Keywords:cow recessive mastitis  Escherichia coli  susceptibility test
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