|
|||||
|
|
| 贵州地方黄牛不同组织中PPARγ基因表达水平研究 | |
| 引用本文： | 张青青,许厚强,陈伟,陈祥,周迪,夏丹,赵焕平,孙成娟,王圆圆,杨洋,张鸣.贵州地方黄牛不同组织中PPARγ基因表达水平研究[J].中国畜牧兽医,2017,44(1):38-43. |
| 作者姓名： | 张青青 许厚强 陈伟 陈祥 周迪 夏丹 赵焕平 孙成娟 王圆圆 杨洋 张鸣 |
| 作者单位： | 1. 高原山地动物遗传育种与繁殖省部共建教育部重点实验室, 贵阳 550025; 2. 贵州省动物遗传育种与繁殖重点实验室, 贵阳 550025; 3. 贵州大学动物科学学院, 贵阳 550025; 4. 贵州大学生命科学学院, 贵阳 550025 |
| 基金项目： | 国家自然科学基金面上项目（31571279）；贵州省科技厅农业攻关项目（黔科合NY字[2012]3008号） |
| 摘 要： | 试验旨在研究威宁牛、思南牛、关岭牛和黎平牛不同组织中过氧化物酶体增殖物激活受体γ（peroxisome proliferators-activated receptors γ，PPARγ）基因mRNA的表达差异。以这4个贵州地方品种黄牛为试验动物，提取心脏、肝脏、脾脏、肺脏、肾脏、脂肪和背最长肌组织的总RNA，设计PPARγ基因的实时荧光定量引物，以牛GAPDH基因为内参，应用实时荧光定量PCR技术检测PPARγ基因在4个品种不同组织中mRNA的相对表达量。结果显示，PPARγ基因在4个品种黄牛的心脏、肝脏、脾脏、肺脏、肾脏、脂肪和背最长肌组织中均有表达，但在脂肪组织中的表达量高于其他组织，且差异极显著（P<0.01），在脾脏和肺脏中也有较高表达，而在肾脏、肝脏、心脏和背最长肌中表达量较低；不同品种PPARγ基因的表达在部分组织中存在差异。研究表明，PPARγ基因在不同组织中的表达量存在差异，可能与其在不同组织中的功能有关，而品种对于PPARγ基因的表达影响不大。 |
| 关 键 词： | 黄牛 PPAR&gamma 基因 表达 实时荧光定量PCR |
| 收稿时间： | 2016-06-23 |
The Expression Level of PPARγ Gene in Different Tissues of Guizhou Cattle Resouces |
|
| ZHANG Qing-qing,XU Hou-qiang,CHEN Wei,CHEN Xiang,ZHOU Di,XIA Dan,ZHAO Huan-ping,SUN Cheng-juan,WANG Yuan-yuan,YANG Yang,ZHANG Ming.The Expression Level of PPARγ Gene in Different Tissues of Guizhou Cattle Resouces[J].China Animal Husbandry & Veterinary Medicine,2017,44(1):38-43. | |
| Authors: | ZHANG Qing-qing XU Hou-qiang CHEN Wei CHEN Xiang ZHOU Di XIA Dan ZHAO Huan-ping SUN Cheng-juan WANG Yuan-yuan YANG Yang ZHANG Ming |
| Institution: | 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guiyang 550025, China; 2. Key Laboratory of Animal Genetics, Breeding and Reproduction in Guizhou, Guiyang 550025, China; 3. College of Animal Science, Guizhou University, Guiyang 550025, China; 4. College of Life Science, Guizhou University, Guiyang 550025, China |
| Abstract: | The objective of this study was to investigate the expression level of PPARγ gene mRNA in different tissues from Weining cattle, Sinan cattle, Guanling cattle and Liping cattle in Guizhou province. Total RNA was extracted from the heart, liver, spleen, lung, kidney, adipose and longissimus dorsi of the cattle resources. Primers of PPARγ gene were designed. The developmental changes of PPARγ gene mRNA relative expression in different tissues from 4 kinds of cattle breeds were detected by Real-time PCR, taking GAPDH gene as the internal reference. The results showed that PPARγ gene was expressed in all tissues. The mRNA expression in adipose tissue was extremely significantly higher than that in other tissues (P<0.01), with a higher expression in the spleen and lung as well, while a lower expression in the kidney, liver, heart and longissimus dorsi.The expression of PPARγ gene in the different cattle resouces was different in partial tissues. The results suggested that PPARγ gene mRNA expression existed differences in the tissues,which might be due to its function in different tissues, but different cattle resouces did not basically influence the PPARγ gene mRNA expression. |
| Keywords: | cattle PPARγ gene expression Real-time PCR |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 | |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 | |