Isolation,Identification and Genotyping of Pasteurella multocida from Fowl Cholera |
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| Authors: | TANG Sha YUAN Hai-wen YANG Yuan ZHANG Yun-dan WANG Jun CHENG Zhen-tao TANG Ying-xiu CHEN Bo |
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| Institution: | 1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Diseases and Veterinary Public Health in Guizhou Province, Guiyang 550025, China;
3. Changshun County Animal Disease Prevention and Control Center, Changshun 550700, China |
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| Abstract: | In order to determine the suspected avian cholera pathogen and geneotypes, bacteria isolation technology was used to isolate and culture pathogenic bacteria, the isolated bacteria were identified by traditional methods and molecular biological methods. Geneotyping of the isolated bacteria were determined using PCR technology and gene sequence analysis. The results demonstrated that the isolated bacteria had typical culture characteristics of Pasteurella multocida (Pm),and the morphology of the colony, the characteristics of cell staining, the physiological and biochemical characteristics were consist with Pm; 457 bp fragment was amplified by PCR. Geneotyping results showed that only A primer amplified the target gene fragment of 1 050 bp, sequence analysis also showed that the isolated bacteria shared 97.6% to 100.0% sequence homology with reference strains, and phylogenetic tree analysis showed that the isolated bacteria and A type Pm were in a branch.The experimental results indicated that the isolated bacteria was identified as capsular serotype A of Pm, the results provided reference for the prevention and control of fowl cholera. |
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| Keywords: | fowl cholera Pasteurella multocida isolation and identification genotyping |
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