Extraction,Purification and Determination of Capsular Polysaccharide in Enterococcus faecium |
| |
| Authors: | LIU Yong-an LI Xue-rui ZHOU Jian-hua MA Li-na LIU Yong-sheng |
| |
| Institution: | Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China |
| |
| Abstract: | In order to extract and purify the capsular polysaccharide of Enterococcus faecium and measure contents of polysaccharide, high pressure crushing methods and enzyme decomposition methods (RNase A (25 μg/mL), DNase (0.5 mg/mL), lysozyme (0.25 mg/mL) and protease K (1.25 mg/mL)) were used to remove the nucleic acids and proteins to obtain crude capsular polysaccharide, and centrifugal ultrafiltration with 30 and 100 ku ultrafiltration centrifuge tubes and DEAE Sepharose Fast Flow column chromatography were also adopted to obtain the purified capsular polysaccharides of Enterococcus faecium. Then the content of polysaccharide was determined by the phenol-sulfuric acid method. The results showed the capsular polysaccharide content was 75.21%. The results revealed that capsular polysaccharide ofEnterococcus faecium was extracted successfully. The method of extracting capsular polysaccharide in Enterococcus faecium was established initially. |
| |
| Keywords: | Enterococcus faecium capsular polysaccharide extraction and purification phenol-sulfuric acid method |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
