Isolation,Identification and Drug Resistance Analysis of Interstinal Pathogenic Escherichia coli and Salmonella Isolated from Diarrhea Piglets |
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| Authors: | LI Chen ZHANG Shuang-xiang ZHOU Bi-jun CHENG Zhen-tao WEN Ming MA Guang-qiang WANG Wei HU Xing-yi ZHANG Hai DING Zun-e WANG Kai-gong |
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| Institution: | 1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Guizhou Province Center for Animal Disease Control and Prevention, Guiyang 550001, China;
3. The Agriculture Bureau of Shuicheng, Liupanshui 553000, China |
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| Abstract: | In order to find out the serotype, resistant phenotype and genotype of Escherichia coli (E. coli) and Salmonella in piglets, this study collected 128 samples of diarrhea piglets from seven large-scale pig farms in five cities in Guizhou province, and the E. coli and Salmonella were isolated and identified. The pathogenicity of the strain was identified by animal test. The drug resistance of the main pathogen was tested by drug susceptibility paper. The resistance gene of each pathogen was detected by PCR. The drug resistance and genotype correlation of the bacterial were analyzed. The results showed that 78 strains of pathogenic E. coli and 21 strains of Salmonella were isolated and identified in this study. The serotypes of pathogenic E. coli were predominantly O138 and O87. Salmonella Typhimurium and Salmonella Enteritidis were predominant serotypes. The susceptibility test showed that the resistant strains of 78 strains of E. coli were more than 80% resistant to β-lactams and more than 40% for other antibacterials. The resistance rate of 21 strains of Salmonella to aminoglycosides was more than 50% and more than 20% to other types of antibacterials; 12 and 10 kinds of drug resistance-related genes of E. coli and Salmonella were detected, respectively; The coincidence rate of resistant genotype and phenotype of two kinds of bacteria were above 60%, and both were multiple drug resistance. This study provided a theoretical basis for comprehensive prevention and control of piglets diarrhea. |
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| Keywords: | Escherichia coli Salmonella isolation and identification drug resistance |
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