Isolation,Identification and Genetic Evolution Analysis of Mink Serratia marcescens |
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| Authors: | PAN Yue WEN Shan-shan ZHAO Li-li YANG Yan LI Si-yu ZHANG Cheng-hao GE Jun-wei CHEN Hong-yan |
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| Institution: | 1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;
2. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China |
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| Abstract: | In order to determine drug resistance, pathogenicity and genetic of the pathogenic bacteria in mink of Heilongjiang province, three strains were isolated from dead minks and identified. Based on colony morphology, microscopic characteristics, biochemical test and 16S rRNA sequencing. The antibiotic susceptibility to 24 antibiotics were detected by using standard K-B paper method, and virulence assay, phylogenetic analysis were carried out at the same time. The result showed that the isolates were highly tolerance to antibacterials, could cause 100% death of mice in experimental group and were closely related to strains from activated sludge and volcanic deposits. It showed that Serratia marcescens infection of mink existed in Heilongjiang province. Strains resistance phenomenon was serious and had a strong pathogenicity, and might be originated from the environment. It was the first time that the pathogenic Serratia marcescens isolated from the mink was reported. The results provided information for the detection, identification, diagnosis, treatment and prevention of related diseases. |
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| Keywords: | mink Serratia marcescens isolation and identification genetic evolution |
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