| 广西地方禽类品种ALV和REV感染情况的调查及ALV分离株gp85基因序列分析 |
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| 引用本文： | 钟兴福,王培坤,杨永立,秦丽莉,毕玉彧,邹广珍,彭昊,韦平.广西地方禽类品种ALV和REV感染情况的调查及ALV分离株gp85基因序列分析[J].中国畜牧兽医,2015,42(7):1647-1653. |
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| 作者姓名： | 钟兴福 王培坤 杨永立 秦丽莉 毕玉彧 邹广珍 彭昊 韦平 |
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| 作者单位： | 1. 广西大学, 养禽与禽病学研究所, 南宁 530004;2. 广西钦州市钦南区沙埠镇水产畜牧兽医站, 钦州 535000 |
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| 基金项目： | 国家公益性(农业)行业科研专项(201203055);国家现代农业产业技术体系广西肉鸡产业创新团队建设项目(nycytxgxcxtd-04-20-2) |
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| 摘 要： | 为了解广西钦州地区主要地方品种商品禽类中禽白血病病毒(ALV)、禽网状内皮组织增殖症病毒(REV)的感染情况,本试验随机采集了广西钦州地区代表饲养场的麻鸭、狮头鹅、铁脚麻鸡、火鸡、鸽子共5个品种的蛋清、肛拭子及血清样品共953份,使用ELISA商品试剂盒进行检测;然后对部分ALV p27阳性的个体采集其血清样品接种DF-1细胞进行病毒分离并测定其gp85基因的序列。结果发现,除铁脚麻鸡外其他4个非鸡禽类品种的ALV检测均为阴性;除鸽子和狮头鹅外,麻鸭、铁脚麻鸡、火鸡均检测到REV抗体阳性;获得的两株铁脚麻鸡ALV分离株gp85基因之间核苷酸同源性为94.5%,与参考株之间核苷酸同源性为86.9%~94.9%;两株分离株gp85基因之间氨基酸同源性为91.5%,与参考株之间氨基酸同源性为84.0%~91.6%。高变区hr1和hr2区存在较多可变位点,可变区vr2和vr3相对较保守。系统进化树分析结果表明这两个毒株与参考株SCAU11-XG的亲缘关系最近。
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| 关 键 词： | 禽类 禽白血病 禽网状内皮组织增殖症 流行病学调查 gp85基因 系统进化树分析 |
Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates |
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| ZHONG Xing-fu,WANG Pei-kun,YANG Yong-li,QIN Li-li,BI Yu-yu,ZOU Guang-zhen,PENG Hao,WEI Ping.Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates[J].China Animal Husbandry & Veterinary Medicine,2015,42(7):1647-1653. |
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| Authors: | ZHONG Xing-fu WANG Pei-kun YANG Yong-li QIN Li-li BI Yu-yu ZOU Guang-zhen PENG Hao WEI Ping |
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| Institution: | 1. Institute of Poultry Science and Health, Guangxi University, Nanning 530004, China;2. Shabu Aquatic Animal Husbandry and Veterinary Station of Qinnan District, Qinzhou 535000, China |
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| Abstract: | In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain. |
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| Keywords: | avian avian leukosis avian reticuloendotheliosis epidemiological survey gp85 gene phylogenetic tree analysis |
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