| 禽大肠杆菌的分离与16S rRNA的鉴定 |
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| 引用本文： | 师福山,赵德明.禽大肠杆菌的分离与16S rRNA的鉴定[J].中国畜牧兽医,2009,36(2):111-113. |
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| 作者姓名： | 师福山 赵德明 |
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| 作者单位： | （中国农业大学动物医学院国家动物海绵状脑病实验室， 北京 100193） |
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| 基金项目： | 科技部资助资助项目，北京市科委资助项目 |
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| 摘 要： | 从疑似患有大肠杆菌病的病死鸡群中采取粪便样品，分离病原进行生化鉴定，从8份样品中分离鉴定出6株大肠杆菌。根据细菌16S rRNA 基因的高度保守性,设计合成大肠杆菌的共同引物,对随机选取的1株细菌进行PCR扩增，并与GenBank中的E.coli 16S rRNA进行序列比对,确定这株细菌与大肠杆菌的同源性达99%以上。本方法特异性好,为实验室鉴定大肠杆菌提供了一种简单、容易操作的手段。
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| 关 键 词： | 大肠杆菌 生化鉴定 16S rRNA PCR |
Isolation and Identification of 16S rRNA of Escherichia coli from Chicken |
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| SHI Fu-shan,ZHAO De-ming.Isolation and Identification of 16S rRNA of Escherichia coli from Chicken[J].China Animal Husbandry & Veterinary Medicine,2009,36(2):111-113. |
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| Authors: | SHI Fu-shan ZHAO De-ming |
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| Institution: | (Laboratory of National Animal TSE,College of Veterinary Medicine , China Agricultural University, Beijing 100193 , China) |
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| Abstract: | Feces samples were collected from sick and dead chicken likely caused by Escherichina coli in a chicken farm，thepathogens were isolated and biochemically identificated, 6 bacterium were isolated from 8 feces samples. According to the highly conservative nature of bacterial 16S rRNA gene, a set of primers with broad range for Escherichina coli was designed，and 1 strain of bacteria was detected by polymerase chain reaction (PCR) and compared with 16S rRNA sequence of Escherichina coli from GenBank.The resembling rate can exceed 99%.The established PCR method is specific ,simple , easy, and available to identify Escherichina coli. |
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| Keywords: | 16S rRNA PCR |
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