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酶联免疫法对生鲜乳中黄曲霉毒素M1的快速检测
引用本文:蒙君丽,宋荪阳,郑百芹,齐彪,张建民,葛怀礼,吴颖,李爱军.酶联免疫法对生鲜乳中黄曲霉毒素M1的快速检测[J].中国畜牧兽医,2013,40(Z1):39-41.
作者姓名:蒙君丽  宋荪阳  郑百芹  齐彪  张建民  葛怀礼  吴颖  李爱军
作者单位:1. 唐山市畜牧水产品质量监测中心, 河北唐山 063000;2. 中央农业广播电视学校唐山分校, 河北唐山 063000;3. 唐山科技情报研究所, 河北唐山 063000
基金项目:生鲜牛乳质量安全监测及合理日粮保障技术研究与示范(11221001D)。
摘    要:采用酶联免疫法(ELISA)对生鲜乳中黄曲霉毒素M1进行检测,对比了3个不同厂家的试剂盒检测结果。结果表明,在空白样品中添加浓度为0.5、1.0、2.5 μg/kg时,试剂盒的回收率在93.5%~103.5%。不同厂家的试剂盒测定结果重复性、精确度均较好,说明酶联免疫法能够有效检测生鲜乳中黄曲霉毒素M1

关 键 词:生鲜乳  黄曲霉毒素M1  酶联免疫法  
收稿时间:2013-08-02

ELISA for Rapid Detection of Aflatoxin M1 in Raw Milk
MENG Jun-li,SONG Sun-yang,ZHENG Bai-qin,QI Biao,ZHANG Jian-min,GE Huai-li,WU Ying,LI Ai-jun.ELISA for Rapid Detection of Aflatoxin M1 in Raw Milk[J].China Animal Husbandry & Veterinary Medicine,2013,40(Z1):39-41.
Authors:MENG Jun-li  SONG Sun-yang  ZHENG Bai-qin  QI Biao  ZHANG Jian-min  GE Huai-li  WU Ying  LI Ai-jun
Institution:1. Tangshan Livestock and Aquatic Products Quality Monitoring Center, Tangshan 063000, China;2. The Central Agricultural Broadcasting and Television School of Tangshan Branch, Tangshan 063000, China;3. Tangshan Science and Technology Information Institute Tangshan 063000, China
Abstract:ELISA for the analysis of aflatoxin M1 in raw milk were determined.The experiment compared the results of enzyme-linked immunosorbent assay kits from different manufacturers. The results showed that when the samples were spiked with 0.5,1.0 and 2.5 μg/kg of aflatoxin M1, the recoveries were 93.5% to 103.5% by ELISA. ESLISA is an effective method for detecting of aflatoxin M1 in raw milk.
Keywords:raw milk  aflatoxin M1  ELISA  
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