| QuEChERS-UPLC-MS/MS快速测定生鲜牛乳中的氟喹诺酮类药物残留 |
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| 引用本文： | 曲斌,朱志谦,陆桂萍,蒋天梅,耿士伟,郭良雪.QuEChERS-UPLC-MS/MS快速测定生鲜牛乳中的氟喹诺酮类药物残留[J].中国畜牧兽医,2013,40(Z1):50-54. |
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| 作者姓名： | 曲斌 朱志谦 陆桂萍 蒋天梅 耿士伟 郭良雪 |
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| 作者单位： | 1. 江苏省畜产品质量检验测试中心, 江苏南京 210036;2. 安捷伦科技(中国)有限公司, 上海 200080 |
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| 摘 要： | 本试验建立了生鲜牛乳中8种氟喹诺酮类药物的超高效液相色谱串联质谱的测定方法。生鲜牛乳经QuEChERS萃取剂提取，QuEChERS净化剂净化后，经氮气流吹干浓缩后，以超高效液相色谱串联质谱测定，稳定同位素内标法定量。本方法对氟喹诺酮类药物的测定线性范围为2.0～100 μg/kg。在2.0、10、100 μg/kg低、中、高3个浓度的回收率为80%～120%。批内、批间精密度小于20%。本方法简便、快速、灵敏，适用于生鲜牛乳中氟喹诺酮类药物残留的大批量筛选和定量测定。
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| 关 键 词： | 超高效液相色谱-串联质谱 QuEChERS 氟喹诺酮 生鲜牛乳 残留 |
| 收稿时间： | 2013-05-29 |
Rapid Determination of Fluoroquinolones Residues in Raw Bovine Milk by UPLC-MS/MS via QuEChERS Pretreatment Method |
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| QU Bin,ZHU Zhi-qian,LU Gui-ping,JIANG Tian-mei,GENG Shi-wei,GUO Liang-xue.Rapid Determination of Fluoroquinolones Residues in Raw Bovine Milk by UPLC-MS/MS via QuEChERS Pretreatment Method[J].China Animal Husbandry & Veterinary Medicine,2013,40(Z1):50-54. |
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| Authors: | QU Bin ZHU Zhi-qian LU Gui-ping JIANG Tian-mei GENG Shi-wei GUO Liang-xue |
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| Institution: | 1. Jiangsu Quality Inspection and Testing Center for Animal Products, Nanjing 210036, China;2. Agilent technologies (China) Co., Ltd., Shanghai 200080, China |
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| Abstract: | An UPLC-MS/MS method was developed and established for rapid determination of fluoroquinolones residues in raw bovine milk. The sample was extracted by QuEChERS extraction Kit, and cleaned up by QuEChERS clean-up tube. An aliquot sample of 5 mL was concentrated by nitrogen gas. The analytes were determined by UPLC-MS/MS and quantified by stable isotope internal standard. The results showed good linearity in the ranges of 2.0 to 100 μg/kg for all fluoroquinolones. The method recoveries were between 80% and 120%, and also the precisions were lower than 20%. The method is simple, rapid, and sensitive. It is suitable for screening of fluoroquinolones residues in raw bovine milk and quantitative determination. |
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| Keywords: | UPLC-MS/MS QuEChERS fluoroquinolone bovine milk residue |
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