| 鸡肠道大肠杆菌的分离鉴定及耐药性分析 |
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| 引用本文： | 张俊丰,黄东璋,陈琳,刘俊栋,魏冬霞,管远红,丁小丽,李巨银,颜友荣,周思思,陈新秀.鸡肠道大肠杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2013,40(11):179-184. |
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| 作者姓名： | 张俊丰 黄东璋 陈琳 刘俊栋 魏冬霞 管远红 丁小丽 李巨银 颜友荣 周思思 陈新秀 |
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| 作者单位： | 1. 重庆市畜牧科学研究院, 重庆荣昌 402460;2. 江苏农牧科技职业学院, 江苏泰州 225300 |
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| 基金项目： | 江苏省自然科学基金(BK2008587);江苏农牧科技职业学院重点项目(ZD1202)。 |
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| 摘 要： | 为分析鸡肠道大肠杆菌对常用抗菌药物的耐药性,试验采集了34个鸡直肠拭子样本,分离并鉴定大肠杆菌,采用微量稀释法测定分离菌对卡那霉素、氯霉素、氨苄西林、环丙沙星、头孢曲松钠、庆大霉素、磺胺嘧啶和强力霉素8种药物的敏感性。结果显示,分离并鉴定出34株鸡肠道大肠杆菌,其中4株经鉴定为产H2S大肠杆菌。34株菌对卡那霉素、氯霉素、氨苄西林、环丙沙星、头孢曲松钠、庆大霉素、磺胺嘧啶和强力霉素的MIC50分别为>256、256、>256、16、<0.125、256、>512和16 μg/mL,耐药率依次为100.0%、100.0%、100.0%、70.6%、11.8%、94.1%、100.0%和82.3%。34株菌均为多重耐药菌株,最少可对5种药物耐药,最多可对8种药物耐药,其中耐8种药物菌株百分率为2.9%;耐7种药物菌株百分率为58.8%;耐6种药物菌株百分率为32.4%;耐5种药物菌株百分率为5.9%。试验结果表明,34株鸡肠道大肠杆菌对8种常用抗菌药物的耐药谱广、耐药率高,兽医临床应根据试验结果调整用药方案。
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| 关 键 词： | 鸡 产H2S大肠杆菌 鉴定 耐药 |
| 收稿时间： | 2013-03-27 |
Identification and Drug Resistance Analysis of Chicken Intestinal Escherichia coli |
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| ZHANG Jun-feng,HUANG Dong-zhang,CHEN Lin,LIU Jun-dong,WEI Dong-xia,GUAN Yuan-hong,DING Xiao-li,LI Ju-yin,YAN You-rong,ZHOU Si-si,CHEN Xin-xiu.Identification and Drug Resistance Analysis of Chicken Intestinal Escherichia coli[J].China Animal Husbandry & Veterinary Medicine,2013,40(11):179-184. |
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| Authors: | ZHANG Jun-feng HUANG Dong-zhang CHEN Lin LIU Jun-dong WEI Dong-xia GUAN Yuan-hong DING Xiao-li LI Ju-yin YAN You-rong ZHOU Si-si CHEN Xin-xiu |
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| Institution: | 1. Chongqing Academy of Animal Science, Rongchang 402460, China;2. Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China |
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| Abstract: | Total of 34 chicken rectal swab samples were collected to isolate and identify Escherichia coli. And all isolates were tested for susceptibility to 8 antimicrobial agents by using the broth micro-dilution method. As a result, 34 intestinal Escherichia coli isolates recovered from chicken were identified and 4 of them were hydrogen sulfide producing stains. Their MIC50 to kanamycin, chloramphenicol, ampicillin, cyprofloxacin, ceftriaxone sodium, gentamicin, sulfadiazine and doxycycline were more than 256 μg/mL, equal to 256 μg/mL, more than 256 μg/mL, equal to 16 μg/mL, less than 0.125 μg/mL, equal to 256 μg/mL, more than 512 μg/mL and equal to 16 μg/mL,respectively. And their resistant rates to these 8 antimicrobial agents were ordered as 100.0%, 100.0%, 100.0%, 70.6%, 11.8%, 94.1%, 100.0% and 82.3%, respectively. All isolates were multi-drug resistant strains. The minimal and maximum kinds of antimicrobial agents to 34 Escherichia coli strains were 5 and 8, respectively. The percentages of resistant isolates against 5, 6, 7 and 8 antimicrobial agents were ordered as 5.9%, 32.4%, 58.8% and 2.9%.It was suggested that wide resistant spectrum and high resistant ratio were presented in 34 intestinal Escherichia coli isolates. Medicinal proposals in clinical should be adjusted according to the antimicrobial susceptibility test. |
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| Keywords: | chicken hydrogen sulfide producing Escherichia coli identification resistance |
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