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Screening of Lactobacillus with Highly Antagonistic Activity against Campylobacter
Authors:RAO Lei  HUANG Wu  HUANG Yong-xing  GAO Rui-juan  LUO Kai-jian
Institution:College of Veterinary Medicine, South China Agriculture University, Guangzhou 510642, China
Abstract:Using probiotics replaced antibiotic for the treatment and prevention of animal in the future, and lactic acid bacteria (LAB) were the application of probiotics in the main component. Test was aimed to screen LAB which had high bacteriostatic activity to Campylobacter and tolerance animal gastrointestinal environment. Poultry excrement were acquired from Guangdong different areas, the isolated LAB were dealed with biochemical identification, PCR identification and 16S rDNA sequencing. Meanwhile, we tried to study the antibacterial ability of them against Campylobacter jejuni isolated from chicken, duck and Campylobacter coli from goose, then we further examed the bearing capability of the LAB under acid, bile salts and trypsin. The results showed that Lactobacillus saliva R3 had high bacteriostatic activity and tolerance ability to different birds source Campylobacter. So, Lactobacillus saliva R3 would be one of candidate strains that used as microecologics to treat poultry Campylobacter disease.
Keywords:Campylobacter  lactic acid bacteria  bacteriostatic activity  tolerance  
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