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文昌鸡源大肠杆菌生物被膜表型和耐药谱型相关性研究
引用本文:陈朝喜,贺冬梅.文昌鸡源大肠杆菌生物被膜表型和耐药谱型相关性研究[J].中国畜牧兽医,2016,43(3):814-819.
作者姓名:陈朝喜  贺冬梅
作者单位:西南民族大学生命科学与技术学院, 成都 610041
基金项目:国家民委科研项目(14XNZ024)
摘    要:本试验分别采用微量肉汤稀释法和改良结晶紫法对65株文昌鸡源大肠杆菌进行15种临床常用抗菌药物耐药性检测和生物被膜形成能力鉴定,以了解海南文昌鸡源大肠杆菌耐药谱型和生物被膜表型之间的相关性。结果显示,65株文昌鸡源大肠杆菌中89.23%具有多重耐药现象,64.62%具有交叉耐药现象;81.54%的菌株具有不同的生物被膜形成能力,只有18.46%的菌株无细菌生物被膜形成能力。本试验结果表明,具有生物被膜形成能力的菌株大都表现出多重耐药性,但交叉耐药性与无生物被膜形成能力菌株相差不明显。

关 键 词:文昌鸡  大肠杆菌  微量肉汤稀释法  生物被膜  
收稿时间:2015-08-10

Correlation Study between Biofilm Phenotype and Drug Resistance Patterns of Escherichia coli Isolated from Wenchang Chicken
CHEN Chao-xi,HE Dong-mei.Correlation Study between Biofilm Phenotype and Drug Resistance Patterns of Escherichia coli Isolated from Wenchang Chicken[J].China Animal Husbandry & Veterinary Medicine,2016,43(3):814-819.
Authors:CHEN Chao-xi  HE Dong-mei
Institution:College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
Abstract:To understand the correlation between drug resistance patterns and biofilm phenotype of Escherichia coli isolated from Wenchang chicken to fifteen drugs,broth microdilution method and improved crystal violet method were used for drug resistance and biofilm-forming ability analysis of 65 Escherichia coli isolated from Wenchang chicken to 15 commonly used drugs.The results showed that 89.23% of the strains were multidrug resistant and 64.62% of which revealed cross resistance;81.54% of the strains had different biofilm-forming ability and only 18.46% of which had no biofilm-forming ability.The results suggested that most of the strains with biofilm-forming ability were multidrug resistant,but there was no significant difference between the strains with biofilm-forming ability and those performed no biofilm-forming ability.
Keywords:Wenchang chicken  Escherichia coli  broth microdilution method  biofilm  
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