| 不同地区3个奶牛场临床型乳房炎细菌病原种类分析 |
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| 引用本文： | 徐玉薇,罗小青,魏学良,陈宏莉,石神炳.不同地区3个奶牛场临床型乳房炎细菌病原种类分析[J].中国畜牧兽医,2016,43(2):548-552. |
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| 作者姓名： | 徐玉薇 罗小青 魏学良 陈宏莉 石神炳 |
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| 作者单位： | 1. 西南大学动物科技学院, 北碚 400715;2. 新疆西部牧业股份有限公司, 新疆 830000;3. 福建长富乳业集团股份有限公司, 福建 365000 |
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| 基金项目： | 科技部国家科技支撑计划课题"原料奶质量安全保障技术集成与示范"(2012BAD43B03) |
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| 摘 要： | 本试验通过对不同地区3个奶牛场临床型乳房炎细菌病原的分离鉴定,分析其种类和感染情况,并结合牧场环境状况和管理方式,提出控制乳房炎的建议.从中国福建、新疆、重庆3个不同规模化奶牛场采集61份临床型乳房炎乳样,采用传统微生物鉴定方法与PCR技术进行细菌分离鉴定.结果表明,61份临床型乳房炎乳样中单一感染44份(72.13%),混合感染15份(24.59%);共分离到细菌80株,经传统微生物鉴定方法与PCR技术鉴定主要为大肠杆菌、葡萄球菌和链球菌,其中传染性微生物13株(16.25%)、环境性微生物46株(57.50%)、机会性微生物11株(13.75%)、其他微生物10株(12.50%);3个奶牛场临床型乳房炎仍以单一感染为主,混合感染占有一定的比例,传染性致病菌和环境性致病菌为主要致病菌.在实际生产中,规模化牧场可以通过对乳房炎病原菌的分离鉴定来摸清牧场奶牛乳房炎的感染情况和致病菌种类,结合生产实际有助于了解牛群管理情况,发现问题并加以改善,更好的控制乳房炎的发生.
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| 关 键 词： | 奶牛 临床型乳房炎 细菌鉴定 种类分析 |
| 收稿时间： | 2015-08-13 |
Analysis of Clinical Mastitis Pathogenic Bacterial Species in Three Dairy Farms of Different Areas |
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| XU Yu-wei,LUO Xiao-qing,WEI Xue-liang,CHEN Hong-li,SHI Shen-bing.Analysis of Clinical Mastitis Pathogenic Bacterial Species in Three Dairy Farms of Different Areas[J].China Animal Husbandry & Veterinary Medicine,2016,43(2):548-552. |
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| Authors: | XU Yu-wei LUO Xiao-qing WEI Xue-liang CHEN Hong-li SHI Shen-bing |
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| Institution: | 1. College of Animal Science and Technology, Southwest University, Beibei 400715, China;2. Xinjiang Western Animal Husbandry Co., Ltd., Xinjiang 830000, China;3. Fujian Changfu Dairy Group Co., Ltd., Fujian 365000, China |
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| Abstract: | In order to control mastitis,clinical mastitis pathogenic bacterial species of three dairy farms in different areas were isolated and identified.The types and infection condition of pathogenic bacteria,environmental conditions and management methods of the farms were analyzed.61 clinical mastitis milk samples from three different large-scale dairy farms in Fujian,Xinjiang,and Chongqing were collected for bacterial isolation and identification using traditional microbial identification methods and PCR technology.Among clinical mastitis milk samples collected in this trial,single infection sample was 44 (72.13%)and mixed infections sample was 15 (24.59%).80 bacteria were isolated,mainly E.coli,Staphylococcus and Streptococcus,including 13 strains of infectious microorganisms (16.25%),46 strains of environmental microorganisms (57.50%),11 strains of chance of microorganisms (13.75%),10 strains of other microorganisms (12.50%).Clinical mastitis in three dairy farms were still given priority to single infection,and mixed infection occupies certain proportion,with infectious pathogens and environmental pathogens as the main pathogenic.In the actual production the dairy cow mastitis infection and pathogen species could be carried out through the isolation and identification of mastitis pathogens in large-scale dairy farms.It was helpful to identify the herd management problems and make improvements to better control the occurrence of mastitis combining with the actual production conditions. |
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| Keywords: | dairy cows clinical mastitis bacterial identification species analysis |
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