| 猪、鸡源分离粪肠球菌的核糖体分型及耐药性分析 |
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| 引用本文： | 钱晓璐,商军,田恺,张浩然.猪、鸡源分离粪肠球菌的核糖体分型及耐药性分析[J].中国畜牧兽医,2016,43(8):2176-2182. |
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| 作者姓名： | 钱晓璐 商军 田恺 张浩然 |
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| 作者单位： | 上海市兽药饲料检测所, 上海 201103 |
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| 基金项目： | 上海市科技兴农重点攻关项目（沪农科攻字（2015）第6-3-1号） |
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| 摘 要： | 对上海地区1个规模化养猪场和1个规模化养鸡场分离鉴定的40株粪肠球菌，采用微量肉汤稀释法对12种抗菌药物进行药敏试验，采用Riboprinter®微生物基因指纹鉴定系统检测分离菌株的基因指纹图谱，分别检测分离菌株中的核糖体型和耐药性。结果表明，在40株粪肠球菌中，80%以上为多重耐药菌株，有4株猪源粪肠球菌对万古霉素耐药，其中有2株表现为对万古霉素高度耐药（MIC>64 mg/L），且对链霉素和庆大霉素同时表现为高度耐药（MIC>2 048 mg/L）。从耐药表型和核糖体分型共同分析粪肠球菌的耐药性，发现多重耐药性严重，同一核糖体型菌株的耐药表型并非完全一致。
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| 关 键 词： | 粪肠球菌 耐药性 核糖体分型 药敏试验 |
| 收稿时间： | 2016-01-25 |
Analysis of Ribosome Genotyping and Antimicrobial Resistance in Enterococcus faecalis Isolated from Pigs and Chickens |
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| QIAN Xiao-lu,SHANG Jun,TIAN Kai,ZHANG Hao-ran.Analysis of Ribosome Genotyping and Antimicrobial Resistance in Enterococcus faecalis Isolated from Pigs and Chickens[J].China Animal Husbandry & Veterinary Medicine,2016,43(8):2176-2182. |
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| Authors: | QIAN Xiao-lu SHANG Jun TIAN Kai ZHANG Hao-ran |
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| Institution: | Shanghai Institute for Veterinary Drug & Feeds Control, Shanghai 201103, China |
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| Abstract: | This study was to investigate the ribosome genotyping and antimicrobial resistance in Enterococcus faecalis strains isolated from 2 large-scale farms in Shanghai.40 isolates were evaluated for their sensitivity to 12 antimicrobial agents by broth microdilution,and the ribosome genotyping (ribotype) was characterized by the Riboprinter® Microbial Characterization System.The resistance rates of most antimicrobial drug were relatively high and multidrug resistant strains were detected with more than 80%.4 strains of Enterococcus faecalis isolated from pigs were resistant to vancomycin,including 2 strains of vancomycin highly resistant (MIC>64 mg/L) with the same time highly resistant to gentamicin and streptomycin (MIC>2 048 mg/L).The results showed that multidrug resistance of Enterococcus faecalis was a serious issue,and the resistant phenotypes of the same type of ribotype was not entirely consistent. |
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| Keywords: | Enterococcus faecalis antimicrobial resistance ribosome genotyping antimicrobial susceptibility test |
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