| 鸭源致病性大肠杆菌耐氟苯尼考floR基因的克隆与序列分析 |
| |
| 引用本文： | 于学辉,李旭玲,杨发龙,海泉.鸭源致病性大肠杆菌耐氟苯尼考floR基因的克隆与序列分析[J].中国畜牧兽医,2010,37(3):84-87. |
| |
| 作者姓名： | 于学辉 李旭玲 杨发龙 海泉 |
| |
| 作者单位： | 西南民族大学生命科学与技术学院,成都,610041 |
| |
| 基金项目： | 国家科技支撑计划(2006BAD06A11);;国家自然科技资源平台建设项目(2005DKA21205-11) |
| |
| 摘 要： | 为调查鸭源致病性大肠杆菌氟苯尼考耐药基因floR的存在情况,利用PCR对20株临床分离的耐氟苯尼考的致病性大肠杆菌进行floR分子检测,分子检测结果显示,全部菌株floR基因阳性;对其中2株大肠杆菌的氟苯尼考耐药基因floR进行了克隆和测序,结果表明,鸭源大肠杆菌floR基因片段的克隆测序结果与预期所得片段结果相符,长度为753 bp,2株鸭源大肠杆菌floR基因的同源性为99.6%,与牛源、鸡源等floR基因的同源性为84.8%~99.9%。系统发育分析发现,2株鸭源大肠杆菌floR基因不在同一支上,亲缘关系较远,表明floR基因的亲缘关系与该基因的来源动物无关。
|
| 关 键 词： | 鸭 致病性大肠杆菌 氟苯尼考 floR基因 克隆测序 |
Cloning and Sequence Analysis of floR Genes of Pathogenic Escherichia coli in Ducks |
| |
| YU Xue-hui,LI Xu-ling,YANG Fa-long,HAI Quan.Cloning and Sequence Analysis of floR Genes of Pathogenic Escherichia coli in Ducks[J].China Animal Husbandry & Veterinary Medicine,2010,37(3):84-87. |
| |
| Authors: | YU Xue-hui LI Xu-ling YANG Fa-long HAI Quan |
| |
| Institution: | College of Life Science and Technology;Southwest University for Nationalities;Chengdu 610041;China |
| |
| Abstract: | In order to investigate the distribution of Florfenicol resistance gene floR in pathogenic E.coli isolates from ducks,the PCR method was used to detect the floR in 20 duck-origin E.coli isolates which showed resistance to Florfenicol.As a result,all 20 isolates are floR positive and a PCR fragment with predicted size of 753 bp was obtained.For confirmationand phylogenetic analysis,floR genes from 2 strains were sequenced.The results demonstrated that the homology between two strains was 99.6%,while differen... |
| |
| Keywords: | duck pathogenic E coli Florfenicol floR gene cloning and sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
