| 华中地区鸡源沙门菌分离鉴定及耐药性分析 |
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| 引用本文： | 王迪轩,高东阳,张天姿,刘维,周祖涛,徐晓娟,蔡旭旺.华中地区鸡源沙门菌分离鉴定及耐药性分析[J].中国畜牧兽医,2019,46(3):931-939. |
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| 作者姓名： | 王迪轩 高东阳 张天姿 刘维 周祖涛 徐晓娟 蔡旭旺 |
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| 作者单位： | 华中农业大学动物医学院, 农业微生物学国家重点实验室, 武汉 430070 |
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| 基金项目： | 国家重点研发计划（2016YFD0501607） |
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| 摘 要： | 为了探明华中地区种鸡场沙门菌（Salmonella）的优势血清型和耐药情况，本研究从湖北、河南、湖南等省市22个规模化鸡场采集病鸡、死胚及弱雏组织样品3 724份，通过分离培养、生化试验、PCR鉴定及血清型试验确定分离菌种属及其血清型，并采用Kirby-Bauer法对分离菌株进行了耐药性分析。结果显示，本试验从3 724份病料中共分离鉴定出124株沙门菌，其中79株为D群肠炎沙门菌（63.71%，79/124），34株为D群鸡白痢沙门菌（27.42%，34/124），8株为B群鼠伤寒沙门菌（6.45%，8/124），有3株沙门菌未能确定血清型。O抗原鉴定79株肠炎沙门菌和34株鸡白痢沙门菌为O9，8株鼠伤寒沙门菌为O4。H抗原鉴定79株肠炎沙门菌为Hg，m，8株鼠伤寒沙门菌为Hi。药敏试验结果显示，124株分离菌株对萘啶酸、氨苄青霉素、四环素和多西环素耐药率分别为95.97%（119/124）、91.94%（114/124）、57.26%（71/124）和70.16%（87/124）；对复方新诺明和红霉素耐药率分别为25.81%（32/124）和12.10%（15/124）；对氯霉素、庆大霉素、头孢噻肟和卡那霉素耐药率分别为6.45%（8/124）、1.61%（2/124）、1.61%（2/124）和0.81%（1/124）；对左氧氟沙星、阿米卡星和多黏菌素B完全敏感。99.19%（123/124）的分离株至少对一种药物耐药，87.10%（108/124）的分离株表现多重耐药。本研究为华中地区养鸡场沙门菌的诊断及防控提供了数据支撑。
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| 关 键 词： | 沙门菌 分离鉴定 血清型 耐药性 |
| 收稿时间： | 2018-09-28 |
Isolation,Identification and Drug Resistance Analysis of Avian Salmonella in Central China |
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| WANG Dixuan,GAO Dongyang,ZHANG Tianzi,LIU Wei,ZHOU Zutao,XU Xiaojuan,CAI Xuwang.Isolation,Identification and Drug Resistance Analysis of Avian Salmonella in Central China[J].China Animal Husbandry & Veterinary Medicine,2019,46(3):931-939. |
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| Authors: | WANG Dixuan GAO Dongyang ZHANG Tianzi LIU Wei ZHOU Zutao XU Xiaojuan CAI Xuwang |
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| Institution: | State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | In order to explore the dominant serovars and antimicrobial resistance of avian Salmonella spp.in Central China,a total of 3 724 issue samples were collected from diseased chickens,dead embryos or weak chick in 22 large-scale farms in Hubei,Henan and Hunan,etc.The isolates and their serotypes were identified by PCR and serotype assay.Kirby-Bauer method was used to analyze the drug resistance of the isolates.The results showed that 124 Salmonella were identified from 3 724 samples,among those 79 strains were Salmonella Enteritidis of D serogroup (63.71%,79/124),34 strains were Salmonella Pullorum of D serogroup (27.42%,34/124),8 strains were Salmonella Typhimurium of B serogroup (6.45%,8/124),whereas 3 strains were failed to be determined.The O antigen of 79 Salmonella Enteritidis and 34 Salmonella Pullorum strains were identified O9 antigen,that of 8 Salmonella Typhimurium strains were O4 antigen.While the H antigen of Salmonella Enteritidis and Salmonella Pullorum strains were identified Hg,m,that of Salmonella Typhimurium strains were Hi.The results of antimicrobial susceptibility test showed that the resistance rate of isolates to nalidixic acid,ampicillin,tetracycline and doxycycline were 95.97% (119/124),91.94% (114/124),57.26% (71/124) and 70.16% (87/124),respectively.The resistance rate to Timethoprim/sulfamethoxazole and erythromycin were 25.81% (32/124) and 12.10% (15/124).The resistance rate to chloramphenicol,gentamicin,cefotaxime and kanamycin were 6.45% (8/124),1.61% (2/124),1.61% (2/124) and 0.81% (1/124),respectively,whereas all isolates were completely sensitive to levofloxacin,amikacin and polymyxin B.Altogether,99.19% (123/124) of these isolates were resistant to at least one drug,and 87.10% (108/124) of isolates showed multiple drug resistance.This study provided the basic data for the diagnosis and prevention of Salmonella in poultry farms in Central China. |
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| Keywords: | Salmonella isolation and identification serovar antimicrobial resistance |
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