| 急性冷应激对阿勒泰羊HSP60、HSP70和HSP90基因mRNA表达的影响 |
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| 引用本文： | 魏殿华,高静雯,汪骁轩,张莉,齐亚银.急性冷应激对阿勒泰羊HSP60、HSP70和HSP90基因mRNA表达的影响[J].中国畜牧兽医,2019,46(12):3680-3689. |
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| 作者姓名： | 魏殿华 高静雯 汪骁轩 张莉 齐亚银 |
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| 作者单位： | 石河子大学动物科技学院, 石河子 832000 |
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| 基金项目： | 国家自然科学基金"北疆地区阿勒泰断尾羔羊及杂交种的耐寒性能及体脂能量代谢研究"（31660702）；石河子大学"3152"人才专项（CXRC201705） |
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| 摘 要： | 为研究急性冷应激通过影响热休克蛋白含量的变化对阿勒泰羊抗寒性能、生产性能及抗病性能等的影响，试验设置常温组（15 ℃±2 ℃）与冷应激组（-25 ℃±2 ℃），每组各5只羊，屠宰后分别采集急性冷应激组（冷应激24 h后）和常温组的心脏、肝脏、脾脏和肾脏组织。采用实时荧光定量PCR技术对HSP60、HSP70和HSP90 mRNA含量变化进行检测，并对HSP60、HSP70和HSP90基因进行同源性分析。结果显示，HSP70和HSP90基因与已有绵羊序列同源性高于99%，而HSP60基因与已有波斯金牛序列同源性高于99%；冷刺激后HSP60基因在心脏、肝脏、脾脏和肾脏组织中的表达较常温组均升高，且在肾脏中的表达量差异极显著（P<0.01）；冷刺激后HSP70基因在心脏、肝脏、脾脏和肾脏组织中的表达较常温组均升高，且在肝脏和肾脏组织中的表达量差异极显著（P<0.01）；冷刺激后HSP90基因在心脏、肝脏、脾脏和肾脏组织中的表达较常温组均升高，且在肝脏组织中表达量差异显著（P<0.05），而在脾脏和肾脏组织中的表达量差异极显著（P<0.01）。表明急性冷应激极大程度地刺激了机体的产热机能，通过通路中的产热相关基因的相互调节使其能量代谢发生改变，从而提高细胞生存率，增强机体对环境胁迫的耐受力，能更好地适应环境温度的变化。试验结果为进一步深入研究急性冷应激对阿勒泰羊机体的影响提供一定理论依据。
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| 关 键 词： | 急性冷应激 HSP60基因 HSP70基因 HSP90基因 |
| 收稿时间： | 2019-07-08 |
Effects of Acute Cold Stress on mRNA Expression of HSP60,HSP70 and HSP90 Genes in Altay Sheep |
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| WEI Dianhua,GAO Jingwen,WANG Xiaoxuan,ZHANG Li,QI Yayin.Effects of Acute Cold Stress on mRNA Expression of HSP60,HSP70 and HSP90 Genes in Altay Sheep[J].China Animal Husbandry & Veterinary Medicine,2019,46(12):3680-3689. |
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| Authors: | WEI Dianhua GAO Jingwen WANG Xiaoxuan ZHANG Li QI Yayin |
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| Institution: | College of Animal Science & Technology, Shihezi University, Shihezi 832000, China |
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| Abstract: | In order to study the effects of acute cold stress on the cold resistance,production performance and disease resistance of Altay sheep by affecting the change of heat shock protein content.This experiment was set in the normal temperature group (15 ℃±2 ℃) and the cold stress group (-25 ℃±2 ℃),each group of 5,respectively.The heart,liver,spleen and kidney tissues were collected from the normal temperature group and the acute cold stress group after 24 h.The changes of HSP60,HSP70 and HSP90 mRNA contents were detected by Real-time quantitative PCR and homology analysis of HSP60, HSP70 and HSP90 genes.The results showed that the homology of HSP70 and HSP90 genes with the existing Ovis aries sequences were higher than 99%,and the HSP60 gene had more than 99% homology with the existing Bos taurus sequence.After cold stimulation,the expressions of HSP60 gene in heart,liver,spleen and kidney were higher than those in normal temperature group,and the expression of HSP60 gene in kidney was extremely significant different from that in control group (P<0.01).The expressions of HSP70 gene in heart,liver,spleen and kidney were higher than those in normal temperature group,and the expressions of HSP70 gene in liver and kidney were extremely significant different (P<0.01).The expressions of HSP90 gene in heart,liver,spleen and kidney were higher than those in normal temperature group,and the expression was significantly different in liver (P<0.05),but the expressions difference in spleen and kidney were extremely significant (P<0.01).It demonstrated that acute cold stress greatly stimulates the individual's heat production function,and changed its energy metabolism through the regulation of heat-producing related genes in the pathway,thus improving the survival rate of cells,enhancing the body's tolerance to environmental stress,and better adapting to the changes of environmental temperature.The results of this experiment provided a theoretical basis for further in-depth study of the effects of acute cold stress on Altay sheep. |
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| Keywords: | acute cold stress HSP60 gene HSP70 gene HSP90 gene |
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