| 鸡约氏乳杆菌的分离鉴定及进化分析 |
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| 引用本文： | 张凤华,夏平安,崔保安,张红英,郭国富.鸡约氏乳杆菌的分离鉴定及进化分析[J].中国畜牧兽医,2010,37(1):74-76. |
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| 作者姓名： | 张凤华 夏平安 崔保安 张红英 郭国富 |
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| 作者单位： | （1.河南农业大学牧医工程学院， 郑州 450002； 2.河南出入境检验检疫局， 郑州 450003） |
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| 摘 要： | 从3日龄健康小鸡肠道中分离到1株乳杆菌,用PCR方法从分离菌株扩增16S rRNA基因,获得大小为1340 bp的DNA片段,该片段的核酸序列已提交GenBank,登录号为EU290749。将分离株的16S rRNA基因核苷酸序列与GenBank上其它乳杆菌进行同源性分析并建立进化树。结果表明,分离株的16S rRNA基因核苷酸序列与NCBI公布的鼠约氏乳杆菌分离株(AB295648)的同源性为99.6%,因此该分离菌株被鉴定为鸡约氏乳杆菌(chicken Lactobacillus johnsonii),命名为HN/0711。
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| 关 键 词： | 约氏乳杆菌 分离鉴定 16S rRNA 进化分析 |
Isolation and Identification and Evolution Analysis of Lactobacillus johnsonii in Chicken |
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| ZHANG Feng-hua,XIA Ping-an,CUI Bao-an,ZHANG Hong-ying,GUO Guo-fu.Isolation and Identification and Evolution Analysis of Lactobacillus johnsonii in Chicken[J].China Animal Husbandry & Veterinary Medicine,2010,37(1):74-76. |
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| Authors: | ZHANG Feng-hua XIA Ping-an CUI Bao-an ZHANG Hong-ying GUO Guo-fu |
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| Institution: | (1.Henan Agricultural University, College of Animal Science and Veterinary Medicine, Zhengzhou 450002, China; 2. Henan Entry-Exit Inspection and Quarantine Bureau of PRC, Zhengzhou 450003, China) |
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| Abstract: | A Lactobacillus was isolated from three days chicken's intestinal tract.Then,the 16S rRNA gene was amplified and analyzed by PCR method,and the PCR product was cloned and sequenced.A sequence of 1340 base pair(bp) was obtained from the bacterium,and it was published on the GenBank,the location is EU290749.The sequence homology and evolution relationship between the isolated bacterium and other Lactobacillus were analyzed,its sequence homology was 99.6% with the Lactobacillus johnsonii(AB295648) that was pub... |
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| Keywords: | Lactobacillus isolation and identification 16S rRNA evolution analysis |
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