| 猪化脓隐秘杆菌的分离与鉴定 |
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| 引用本文： | 鲁杏华,何世成,谈志祥,刘道新,邱立新,范仲鑫,唐小明,黄建龙,王洪冰.猪化脓隐秘杆菌的分离与鉴定[J].中国畜牧兽医,2009,36(5):149-151. |
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| 作者姓名： | 鲁杏华 何世成 谈志祥 刘道新 邱立新 范仲鑫 唐小明 黄建龙 王洪冰 |
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| 作者单位： | （湖南省动物疫病预防控制中心， 长沙 410007） |
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| 摘 要： | 从一例肝、肺化脓性结节，脾出血性肿大的病猪体内分离到一株革兰氏阳性、细长、形态不规则的杆菌，该菌在TSA平板、普通绵羊血琼脂、巧克力平板上生长缓慢，在普通绵羊血琼脂上呈α-溶血。对该菌的16S rDNA进行PCR扩增、测序、比较，结果显示该菌与化脓隐秘杆菌的16S rDNA有99%的同源性，进一步用API生化鉴定，结果也与其相符。该菌在24~52 h内100%致死小白鼠，腹腔接种新西兰兔，接种兔72 h内死亡，表明该菌为致病性细菌。
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| 关 键 词： | 化脓隐秘杆菌 16S rDNA PCR 生化鉴定 |
Isolation and Identification of Arcanobacterium Pyogenes of Swine |
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| LU Xing-hua,HE Shi-cheng,TAN Zhi-xiang,LIU Dao-xin,QIU Li-xin,FAN Zhong-xin,TANG Xiao-ming,HUANG Jian-long,WANG Hong-bing.Isolation and Identification of Arcanobacterium Pyogenes of Swine[J].China Animal Husbandry & Veterinary Medicine,2009,36(5):149-151. |
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| Authors: | LU Xing-hua HE Shi-cheng TAN Zhi-xiang LIU Dao-xin QIU Li-xin FAN Zhong-xin TANG Xiao-ming HUANG Jian-long WANG Hong-bing |
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| Institution: | (The Animal Disease Control Center of Hunan Province, Changsha 410007, China) |
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| Abstract: | A bacterial which was Gram positive, slenderness and irregularity in appearance were isolated from a pig， its liver had purulence nodus and lungs and the spleen were haematocele. The isolated bacterium grew slowly in BSA, sheep’s blood agar and chocolate agar, and showed α-haemolysis in sheep’s blood agar. The results of 16S rDNA PCR sequence and API biochemistry identification showed that the isolated bacterium was Arcanobacterium pyogenes. The Arcanobacterium pyogenes caused 100% mouse death in 24~52 h, and cause rabbit death in 72 h by enterocoelia innoculation, it showed that it was virulent bacterium. |
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| Keywords: | 16S rDNA PCR |
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