| 日粮粗蛋白质水平对伊犁鹅产蛋规律、繁殖激素分泌及相关基因mRNA表达的影响 |
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| 引用本文： | 赵晓钰,沙尔山别克·阿不地力大,马晨,吴盈萍,王俊花,李海英.日粮粗蛋白质水平对伊犁鹅产蛋规律、繁殖激素分泌及相关基因mRNA表达的影响[J].中国畜牧兽医,2020,47(6):1729-1738. |
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| 作者姓名： | 赵晓钰 沙尔山别克·阿不地力大 马晨 吴盈萍 王俊花 李海英 |
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| 作者单位： | 新疆农业大学动物科学学院, 乌鲁木齐 830052 |
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| 基金项目： | 新疆维吾尔自治区重点实验室开放课题（2019D04011）；国家自然科学基金项目（31960648） |
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| 摘 要： | 为探讨日粮粗蛋白质水平对伊犁鹅的产蛋规律、繁殖激素分泌及生殖轴相关基因mRNA表达量的影响，本研究随机选取200只3岁伊犁鹅（年龄、饲养管理水平一致及体重相近），随机分为4个组，每组10个重复。对照组及公鹅饲喂鹅场自配料（粗蛋白质水平11.20%），试验组母鹅分别饲喂粗蛋白质水平为13.86%、15.20%和16.48%的日粮，其余营养指标基本一致。预试期1周，正试期9周。结果表明：①试验期内伊犁鹅的产蛋率呈波动变化，对照组伊犁鹅的产蛋率在第1~2周逐渐上升，第4~5周出现小幅度增长，其余阶段表现为下滑趋势；各试验组伊犁鹅的产蛋率在第1~2、4~5周出现较大幅度增长，且在第5周时达到产蛋高峰，此后逐渐下滑。15.20%粗蛋白质组伊犁鹅就巢率显著低于11.20%、13.86%粗蛋白质组。②与对照组相比，13.86%粗蛋白质组血清促性腺激素释放激素（GnRH）浓度极显著升高（P<0.01），且血清雌二醇（E2）、促黄体素（LH）浓度均显著升高（P<0.05）；15.20%、16.48%粗蛋白质组血清GnRH、E2及LH浓度均极显著升高（P<0.01），且血清促卵泡素（FSH）浓度显著升高（P<0.05）。各试验组血清催乳素（PRL）浓度均显著降低（P<0.05）。③与对照组相比，15.20%粗蛋白质组下丘脑GnRH、LHR基因表达量显著上调（P<0.05），PRL、PRLR基因表达量显著下调（P<0.05）；垂体中LH基因表达量显著上调（P<0.05）；卵巢中LH与ESR2基因表达量显著或极显著上调（P<0.05，P<0.01）。综上所述，基于日粮粗蛋白质水平对伊犁鹅的产蛋率、就巢率、血清生殖激素浓度及生殖轴相关基因mRNA相对表达量影响的综合评估，建议产蛋期伊犁鹅日粮粗蛋白质水平为15.20%。
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| 关 键 词： | 粗蛋白质 伊犁鹅 产蛋规律 繁殖激素 mRNA |
| 收稿时间： | 2019-12-02 |
Effects of Dietary Crude Protein Level on Laying Raws,Reproductive Hormone Concentrations and Related Gene mRNA Expression in Reproductive Axis of Yili Geese |
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| ZHAO Xiaoyu,SHAERSHANBIEKE Abudilida,MA Chen,WU Yingping,WANG Junhua,LI Haiying.Effects of Dietary Crude Protein Level on Laying Raws,Reproductive Hormone Concentrations and Related Gene mRNA Expression in Reproductive Axis of Yili Geese[J].China Animal Husbandry & Veterinary Medicine,2020,47(6):1729-1738. |
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| Authors: | ZHAO Xiaoyu SHAERSHANBIEKE Abudilida MA Chen WU Yingping WANG Junhua LI Haiying |
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| Institution: | College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | This experiment was conducted to study the effects of dietary crude protein level on laying raws,reproductive hormone concentrations and related gene mRNA expression in reproductive axis of Yili geese.200 Yili geese aged 3 years old with similar body weight and good health were selected,and were randomly divided into 4 groups with 10 replications.The crude protein level of control group and male geese (goose field self-administered) was 11.20%.The experimental groups fed the test diets with crude protein levels of 13.86%,15.20% and 16.48%,respectively.The nutritional levels of other diets remained the same.The pre-trial period was 1 week,the formal period was 9 weeks.The results showed that:①The egg production rate of Yili geese showed fluctuating changes during the trial period.The egg production rate of Yili geese in control group gradually increased in the first to second weeks,and slightly increased in the fourth to fifth weeks,the other stages showed a downward trend.The egg production rate of Yili geese in each experimental group significantly increased during the first to second and the fourth to fifth weeks,and reached the peak of egg production at the fifth week,and then gradually declined.The broodiness rate of 15.20% crude protein group in Yili geese was significantly lower than that of 11.20% and 13.86% crude protein groups.②Compared with control group,the serum GnRH content of 13.86% crude protein group were extremely significantly increased (P<0.01),and the serum E2 and LH contents significantly increased (P<0.05).The serum GnRH,E2 and LH content of 15.20% and 16.48% crude protein groups were extremely significantly increased (P<0.01),and the serum FSH content was significantly increased (P<0.05).The serum PRL concentration of each test group was significantly lower than that of control group (P<0.05).③Compared with control group,the GnRH,LHR mRNA relative expression of hypothalamus in 15.20% crude protein group were significantly increased (P<0.05),and the PRL and PRLR mRNA relative expression was significantly decreased (P<0.05).The LH mRNA relative expression in pituitary was significantly increased (P<0.05).The LH and ESR2 mRNA relative expression in ovary were significantly or extremely significantly increased (P<0.05 or P<0.01).In summary,based on the comprehensive evaluation of the effects of dietary crude protein level on the laying rate,broodiness rate,serum reproductive hormone concentration and the related gene mRNA expression in reproductive axis of Yili geese.It suggested the suitable crude protein level of the diet in Yili geese was 15.20% during laying period. |
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| Keywords: | crude protein Yili geese laying raws reproductive hormone mRNA |
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