| 鱼源山梨醇发酵阳性温和气单胞菌的分离与鉴定 |
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| 引用本文： | 李旭,丁楚楚,李雪,石慧,陈业翔,胡秀彩,朱爱华,吕爱军.鱼源山梨醇发酵阳性温和气单胞菌的分离与鉴定[J].中国畜牧兽医,2014,41(4):241-244. |
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| 作者姓名： | 李旭 丁楚楚 李雪 石慧 陈业翔 胡秀彩 朱爱华 吕爱军 |
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| 作者单位： | (江苏师范大学生命科学学院, 江苏徐州 221116) |
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| 基金项目： | 国家自然科学基金(31272692、30800847)。 |
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| 摘 要： | 从鲫鱼肠道中分离出1株细菌，暂时编号为SR-1，进行细菌形态学观察、理化特征、16S rDNA序列分析及药敏试验等研究，结果表明SR-1菌株为山梨醇发酵阳性的温和气单胞菌；PCR扩增SR-1菌株的16S rDNA序列为1509 bp；BLAST比对分析结果表明SR-1菌株与温和气单胞菌(Aeromonas sobria)亲缘关系最近，序列同源性为99.87%～100%；药敏试验结果显示SR-1菌株对阿奇霉素、氯霉素、四环素、诺氟沙星、头孢噻肟、头孢克肟等药物敏感。
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| 关 键 词： | 鲫鱼 温和气单胞菌 分离鉴定 药敏试验 系统进化树 |
| 收稿时间： | 2013-09-22 |
Isolation and Identification of Sorbitol-positive Aeromonas sobria from Fish |
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| LI Xu,DING Chu-chu,LI Xue,SHI Hui,CHEN Ye-xiang,HU Xiu-cai,ZHU Ai-hua,LV Ai-jun.Isolation and Identification of Sorbitol-positive Aeromonas sobria from Fish[J].China Animal Husbandry & Veterinary Medicine,2014,41(4):241-244. |
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| Authors: | LI Xu DING Chu-chu LI Xue SHI Hui CHEN Ye-xiang HU Xiu-cai ZHU Ai-hua LV Ai-jun |
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| Institution: | (School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, China) |
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| Abstract: | A bacteria was isolated from the intestine of Carassius auratus and temporarily named as SR-1 strain with sorbitol-positive character. It was identified as Aeromonas sobria by morphological, physiological, biochemical characteristics, drug sennsitivity test, 16S rDNA sequence and phylogenetic tree analysis. Further the 16S rDNA sequence of SR-1 was amplified by PCR method and the results showed that the size of fragment was 1509 bp. Phylogenetic tree analysis showed that SR-1 strain had the highest similarity to Aeromonas sobria of 99.87% to 100%. Drug sensitivity test results revealed that SR-1 was sensitive to azithromycin, chloroamphenicol, tetracycline, norfloxacin, cefotaxime， cefixime and so on. |
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