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猪源奇异变形杆菌的分离鉴定及集群运动分析
引用本文:赵振鹏,杨振,林伟东,于恩琪,李玉,张笛,秦爱建,金文杰.猪源奇异变形杆菌的分离鉴定及集群运动分析[J].中国畜牧兽医,2014,41(10):219-224.
作者姓名:赵振鹏  杨振  林伟东  于恩琪  李玉  张笛  秦爱建  金文杰
作者单位:扬州大学兽医学院, 教育部禽类预防医学重点实验室, 江苏省动物重要疫病与人兽共患病防控协同创新中心, 江苏扬州 225009
基金项目:江苏省“青蓝工程”;江苏高校优势学科建设工程资助项目(PAPD)
摘    要:本试验从发病猪肝脏中分离到1株细菌,经革兰氏染色镜检、生化试验、药敏分析、16S rDNA测序,确认该菌为1株多重耐药奇异变形杆菌。该菌与GenBank中其他9株不同来源的奇异变形杆菌进行16S rDNA比对分析,从遗传进化规律方面证明不同来源的的奇异变形杆菌亲缘性极其相近。周期性群集运动分析结果表明在含0.5%和1.0%琼脂平板上,该菌不以圆环样周期运动;在含1.5%和2.0%琼脂平板上,该菌以圆环样周期运动。

关 键 词:奇异变形杆菌    分离鉴定  周期性群集运动  
收稿时间:2014-04-24

Isolation,Identification and Swarming Motility Analysis of Proteus mirabilis from Pig
ZHAO Zhen-peng,YANG Zhen,LIN Wei-dong,YU En-qi,LI Yu,ZHANG Di,QIN Ai-jian,JIN Wen-jie.Isolation,Identification and Swarming Motility Analysis of Proteus mirabilis from Pig[J].China Animal Husbandry & Veterinary Medicine,2014,41(10):219-224.
Authors:ZHAO Zhen-peng  YANG Zhen  LIN Wei-dong  YU En-qi  LI Yu  ZHANG Di  QIN Ai-jian  JIN Wen-jie
Institution:Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Key Labotory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Abstract:One pathogen was isolated from the liver of the infected pig. The pathogen was identified as multi-resistant Proteus mirabilis by gram staining,microscopy,biochemical,antibiotic resistance tests and 16S rDNA gene sequence analysis. The genetic evolution analysis of 16S rRNA of isolated strain and 9 other strains Proteus mirabilis isolated from different sources proved that the genetic relationship of Proteus mirabilis isolated from different sources was very close in terms of genetic evolution. The swarming motility analysis showed that isolated strain in containing 0.5% or 1.0% agar powder solid medium didn't appeared annular wave motility,and in containing 1.5% and 2.0% agar powder solid medium appeared annular wave motility.
Keywords:Proteus mirabilis  pig  isolation and identification  swarming motility  
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