| 鱼源嗜水气单胞菌对氟苯尼考的耐药性分析 |
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| 引用本文： | 王小亮,徐立蒲,王姝,王静波,曹欢.鱼源嗜水气单胞菌对氟苯尼考的耐药性分析[J].中国畜牧兽医,2014,41(6):216-220. |
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| 作者姓名： | 王小亮 徐立蒲 王姝 王静波 曹欢 |
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| 基金项目： | 北京市农业科技新星项目（嗜水气单胞菌对氟苯尼考的耐药性获得和消失速率研究）；北京市农业科技项目（PXM2012_036237_000012）。 |
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| 摘 要： | 为阐明淡水鱼的主要致病菌嗜水气单胞菌对氟苯尼考的耐药现状，本试验采用纸片扩散法和浓度稀释法分别定性、定量检测了2010年9月至2013年5月期间收集的26株鱼源嗜水气单胞菌对氟苯尼考的耐药性，利用PCR法检测菌株携带氟苯尼考耐药基因的情况。结果显示，北京地区的鱼源嗜水气单胞菌对氟苯尼考的耐药率为15.38%，氟苯尼考对其最小抑菌浓度（MIC）主要集中在2～4 μg/mL，5株菌对氟苯尼考的MIC超过8 μg/mL，1株分离菌具有氟苯尼考耐药基因floR。结合以往数据及其他省市的相关数据，结果表明鱼源嗜水气单胞菌对氟苯尼考已呈较高的耐药率和耐药浓度，且位于质粒上的floR基因阳性菌株有传递该基因给其他种属鱼类致病菌的风险。因此，有必要科学规范氟苯尼考在水产养殖中的应用。
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| 收稿时间： | 2013-11-13 |
Resistance Analysis of Florfenicol to Aeromonas hydrophila from Freshwater Fish |
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| WANG Xiao-liang,XU Li-pu,WANG Shu,WANG Jing-bo,CAO Huan.Resistance Analysis of Florfenicol to Aeromonas hydrophila from Freshwater Fish[J].China Animal Husbandry & Veterinary Medicine,2014,41(6):216-220. |
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| Authors: | WANG Xiao-liang XU Li-pu WANG Shu WANG Jing-bo CAO Huan |
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| Abstract: | To evaluate Aeromonas hydrophila in the status of drug resistance to florfenicol which was the major pathogen from freshwater fish, we tested resistance to florfenicol of 26 strains of Aeromonas hydrophila collected from freshwater fish in Beijing area nearly three years using disk diffusion method and concentration dilution method, respectively. Then, we detected the florfenicol resistance gene of strains using PCR method. The results showed that 15.38% of stains were resistant to florfenicol. The minimum inhibitory concentration of florfenicol to strains was mainly 2 to 4 μg/mL presently, and 5 strains were more than 8 μg/mL. Florfenicol resistance gene floR was detected in 1 strain. Combined with previous data and the relative data of other provinces, it was obvious that the florfenicol resistance rate and resistance concentration of Aeromonas hydrophila were both higher than before, and the strains carried floR gene located in plasmid on the risk of transferring the gene to other species of fish pathogens. Therefore, it was necessary to use florfenicol scientifically in aquaculture. |
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