| 禽巴氏杆菌Pm-HB株的分离与鉴定 |
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| 引用本文： | 尹秀凤,王元珍,丁美娟,许秀梅,黄显明,张海涛,毛火云,张小飞.禽巴氏杆菌Pm-HB株的分离与鉴定[J].中国畜牧兽医,2014,41(5):45-48. |
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| 作者姓名： | 尹秀凤 王元珍 丁美娟 许秀梅 黄显明 张海涛 毛火云 张小飞 |
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| 基金项目： | 江苏省科技成果转化专项资金（BA2010021）。 |
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| 摘 要： | 湖北省某鸡场疑似巴氏杆菌(Pasteurella)感染，从送检病鸡的肺脏和肝脏中分离到1株细菌，通过菌落形态、培养特性、生化试验、16S rRNA序列比对鉴定为禽多杀性巴氏杆菌，并命名为Pm-HB株。动物回归试验表明，10 CFU细菌即可100%致死30日龄SPF鸡，可确诊为巴氏杆菌病。
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| 收稿时间： | 2013-10-16 |
Isolation and Identification of Avian Pasteurella Strain Pm-HB |
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| YIN Xiu-feng,WANG Yuan-zhen,DING Mei-juan,XU Xiu-mei,HUANG Xian-ming,ZHANG Hai-tao,MAO Huo-yun,ZHANG Xiao-fei.Isolation and Identification of Avian Pasteurella Strain Pm-HB[J].China Animal Husbandry & Veterinary Medicine,2014,41(5):45-48. |
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| Authors: | YIN Xiu-feng WANG Yuan-zhen DING Mei-juan XU Xiu-mei HUANG Xian-ming ZHANG Hai-tao MAO Huo-yun ZHANG Xiao-fei |
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| Abstract: | A poultry farm suffered an unknown disease suspected as fowl cholera in Hubei province. A bacterium strain was isolated from liver and lung samples. The isolates was identified as Pasteurella multocida by morphological and cultural characteristics, biochemical properties, and sequence alignment based on 16S rRNA, and named as Pasteurella multocida -HB strain. 30 day old SPF chickens were used to test the pathogenicity and the result showed the strain was deadly to chickens at very low dose, 10 CFU per chicken. |
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