Detection of Resistance to Aminoglycoside Antibiotics and Four Resistance Genes of Canine Escherichia coli |
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| Authors: | WEN Juan YANG Xiao-nong ZHU Zi-feng CHEN Rui YANG Hong-tao RONG Mao-ling |
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| Institution: | 1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;2. The Hospital of Southwest University for Nationalities, Chengdu 610041, China |
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| Abstract: | The objective of this paper was to investigate the drug resistance of canine Escherichia coli (E.coli) strains and the carrying rates of four resistance genes,and explore the relationship between resistance phenotypes and resistance genes.This article chose the aminoglycoside antibiotics including gentamicin,amikacin,spectinomycin and tobramycin to carry out the antibiotics sensitivity test.According to the established PCR assays,we detected the molecular characteristics of the 156 strains of isolates.The positive fragments of four kinds of resistance genes were cloned and sequenced,and the relationships between antibiotics sensitivity test and the resistance genes were analyzed.The results showed that the resistance rates of canine E.coli strains to gentamycin,tobramycin,spectinomycin and amikacin were 55.8%,32.7%,25.0% and 20.5%,respectively.The detection rates of resistance genes aacC2,aphA3, aadA and aacC4 were 55.8%,26.3%,23.1% and 9.0%,respectively.Two strains carried all four kinds of resistance genes,eight strains carried two kinds of resistance genes,and the strains carried two or more resistance genes accounted for 40.4% (63/156).The sequence analysis showed that the amplified gene fragments had higher homology compared with the sequences from GenBank.The main resistance gene in canine E.coli was aacC2 and there was a positive correlation between resistance rates and resistance genes compliance rate. |
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| Keywords: | canine E coli aminoglycosides resistance genes |
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