Isolation,Identification and 16S rDNA Homology Analysis of Escherichia coli from Animals in Different Cases |
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| Authors: | FANG Guang-yuan MAO Hui-hua JIANG Jia-jin GU Ya-feng YAN Wen-mei DAI Ding-zhen GUO Yu-fei |
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| Institution: | College of Animal Science and Technology, Jinling Institute of Technology, Nanjing 210038, China |
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| Abstract: | In order to study the causes of different cases,and analyze 16S rDNA homology of bacteria isolated from different cases,this study took the material of 10 different cases to isolate and culture bacteria,identify the isolates by microbiology.A pair of primers was designed to amplify the bacteria 16S rDNA gene.PCR products were sequenced and 16S rDNA homology analysis was conducted.The results showed that 10 strains bacterial were Escherichia coli through microbiology identification,6 strains of Escherichia coli from mammals cases,16S rDNA homologies among canine skin fester sores,canine pyometra,canine pneumonia,canine mastitis,dairy cow mastitis and calf diarrhea were 100.0% in 10 strains of Escherichia coli,4 strains of Escherichia coli from poultry animal cases,16S rDNA homologies among pigeon diarrhea,broiler diarrhea,pheasant diarrhea and white peacock diarrhea were also 100.0% in 10 strains of Escherichia coli;16S rDNA homologies in Escherichia coli of 10 different cases animal sources were 97.5% to 100.0%.This test proved that 10 different animals cases were caused by Escherichia coli infection,and had a high 16S rDNA homology among 10 strains of Escherichia coli. |
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| Keywords: | animals Escherichia coli isolation and identification 16S rDNA homology analysis |
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