Isolation and Identification of Respiratory Pathogens of Chicken |
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| Authors: | SUN Ming-jie CHEN Lei LIN Xian-hua CUI Bin WANG Jun GU Wei ZHANG Jian-mei |
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| Institution: | Shandong BaoLai-LeeLai Bioengineering Co., Ltd., Tai'an 271000, China |
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| Abstract: | This study was aimed to provide the basis for prevention and control of development of a new product for bacterial respiratory disease of chicken.Diseased samples such as throat, trachea and lungs were inoculated on various selected media for isolating bacteria.17 isolated strains were identified by biochemistry assay and PCR, and then animal experiment were carried out.Black and glassy surface with metallic shine colony from EMB medium Gram-negative bacilli were observed under optical microscope.1 500 bp products were specifically amplified by PCR.DNA sequencing results were analyzed by BLAST and DNAStar with 16S rDNA sequence in GenBank.The results showed that the strains were identified as Escherichia coli.It could cause damage infection with the isolated bacteria through noses.Significant blood spots were observed on throat.Trachea was congestion, swelling and studded with blood points.Heart and liver were wrapped with yellow cheese substances.Air sac was yellow and opaque.The isolated bacteria could provide basis for developing a new product. |
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| Keywords: | chicken respiratory isolation identification |
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