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3株斑羚、岩羊化脓隐秘杆菌的鉴定及系统发育分析
引用本文:杜昕波,赵耘,李伟杰,康凯,陈敏,陈永林.3株斑羚、岩羊化脓隐秘杆菌的鉴定及系统发育分析[J].中国畜牧兽医,2010,37(4):60-62.
作者姓名:杜昕波  赵耘  李伟杰  康凯  陈敏  陈永林
作者单位:(中国兽医药品监察所, 北京 100081)
基金项目:国家自然科技资源平台项目(2005OKA212053)
摘    要:本研究利用Biolog快速鉴定系统1、6S rRNA序列分析及传统细菌鉴定方法对3株分离自北京动物园斑羚、岩羊化脓隐秘杆菌进行了鉴定和系统发育分析。结果表明,3株菌株均为化脓隐秘杆菌;系统发育分析结果表明,3株化脓隐秘杆菌16S rRNA序列与NCTC5224模式株同源性均为100%,且位于系统发育树的同一分支。

关 键 词:化脓隐秘杆菌  Biolog快速鉴定系统  16S  rRNA  鉴定  系统发育分析  

Identification and Phylogenetic Analysis of Arcanobacterium Pyogenes Isolated from Naemorhedus Goral and Pseudois
DU Xin-bo,ZHAO Yun,LI Wei-jie,KANG Kai,CHEN Min,CHEN Yong-lin.Identification and Phylogenetic Analysis of Arcanobacterium Pyogenes Isolated from Naemorhedus Goral and Pseudois[J].China Animal Husbandry & Veterinary Medicine,2010,37(4):60-62.
Authors:DU Xin-bo  ZHAO Yun  LI Wei-jie  KANG Kai  CHEN Min  CHEN Yong-lin
Institution:(China Institute of Veterinery Drug Control, Beijing 100081, China)
Abstract:To identifiy and analyze the three Arcanobacterium pyogenes isolates from Naemorhedus goral and Pseudois,the biochemistry test,analysis of 16S rRNA,automatic micbiology analysis and the other classical methods.The results showed that the three strains were Arcanobacterium pyogenes.The results of phylogenetic analysis showed that the homology of three strains campared with NCTC5224(type strain) were 100%,and they were in the same branch.
Keywords:Arcanobacterium pyogenes  Biolog microbes identification system  16S rRNA  identification  phylogenetic analysis  
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