| 一株猪源棒状杆菌的分离及其16S rRNA基因分析鉴定 |
| |
| 引用本文： | 李敏,田明星,邹年莉,王远萍,柳萍,黄勇.一株猪源棒状杆菌的分离及其16S rRNA基因分析鉴定[J].中国畜牧兽医,2010,37(10):76-80. |
| |
| 作者姓名： | 李敏 田明星 邹年莉 王远萍 柳萍 黄勇 |
| |
| 作者单位： | （1.四川农业大学动物医学院， 雅安 625014；2.四川农业大学动物疫病与人类健康四川省重点实验室， 雅安 625014） |
| |
| 基金项目： | 教育部《长江学者和创新团队发展计划》创新团队项目 |
| |
| 摘 要： | 四川某商品猪场仔猪出现以体温升高,背、腹和腿部皮肤发绀,淋巴结出血肿大,肺、脾脏出血、化脓为主要特征的疾病。通过对分离细菌的纯化培养、形态学观察、生化试验、致病性试验和药物敏感性试验,鉴定出一株致病菌。应用通用引物对其16S rRNA基因进行克隆和序列分析,序列在NCBI上进行BLAST比对,选择同源性较高的棒状杆菌属中25株不同种的菌株16S rRNA序列进行比对分析,构建遗传进化树。分离纯化的细菌在鲜血琼脂平板厌氧条件下生长良好,为革兰氏染色阳性杆状菌,能发酵部分糖类,能致死小白鼠,对头孢类抗生素敏感,16S rRNA序列与棒状杆菌属细菌的同源性最高,在78.2%~98.0%之间,遗传进化树分析结果表明,该菌株与棒状杆菌属细菌属于同一分类群,分离菌鉴定为棒状杆菌。
|
| 关 键 词： | 棒状杆菌 分离 鉴定 16S rRNA 同源性分析 |
Isolation of Swine Corynebacterium and its Identification by Analysis of 16S rRNA Gene |
| |
| LI Min,TIAN Ming-xing,ZOU Nian-li,WANG Yuan-ping,LIU Ping,HUANG Yong.Isolation of Swine Corynebacterium and its Identification by Analysis of 16S rRNA Gene[J].China Animal Husbandry & Veterinary Medicine,2010,37(10):76-80. |
| |
| Authors: | LI Min TIAN Ming-xing ZOU Nian-li WANG Yuan-ping LIU Ping HUANG Yong |
| |
| Institution: | （1.College of Veterinary Medicine,Sichuan Agricultural University, Ya’an 625014, China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, China） |
| |
| Abstract: | An infectious disease mainly characterized of fervescence, cyanosis skin, lymphadenectasis, swollen lungs, splenomegaly, bleeding and purulent occurred in a breeding farm in Ya’an in the province of Sichuan. A pathogen was isolated by blood agar culturing, and characterized by morphological observation, biochemical test, antibiotic sensitive test and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence was compared with sequences of other 25 reference bacteria belonging to Corynebacterium. The results showed that the isolated bacteria was G+, rod-shaped,and sensitive to the cephalosporin antibiotics,the homology analysis showed that the homology between isolated bacterium and other strains of Corynebacterium was from 78.2% to 98.0%. Through phylogenetic analysis, the isolated bacteria was identified as Corynebacterium. |
| |
| Keywords: | 16S rRNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
