| 1株羊源致病性屎肠球菌的分离与鉴定 |
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| 引用本文： | 白伟琴,李宏,孟克,訾占飞,李杰,赵利军,王金玲,格日勒图,李琦,吉林台.1株羊源致病性屎肠球菌的分离与鉴定[J].中国畜牧兽医,2021,48(10):3889-3895. |
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| 作者姓名： | 白伟琴 李宏 孟克 訾占飞 李杰 赵利军 王金玲 格日勒图 李琦 吉林台 |
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| 作者单位： | 1. 内蒙古农业大学兽医学院, 呼和浩特 010018;2. 伊金霍洛旗动物疫病预防控制中心, 鄂尔多斯 017200 |
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| 基金项目： | 内蒙古农业大学高层次人才科研启动金项目（RZ1900003579）；伊金霍洛旗动物疫病预防控制中心采购虫媒本底委托调查和检测、野生动物调查（RH1900007892）；边境地区媒介生物外来动物疫病检测溯源技术研究（2017YFD0501800） |
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| 摘 要： | 鄂尔多斯市某羊场发生羊不明原因死亡的疫情，为了寻找病因并制定治疗方案和防治措施，本研究通过临床检查和实验室病理及分子生物学诊断方法确诊并提供治疗方案。试验以病死羊为试验材料，首先对其进行临床检查，包括尸体剖检和病理组织学采样。随后，采用常规无菌操作方法取回其脑组织至实验室，进行触片、染色镜检和分离细菌。病死羊剖检发现，肺脏淤血实变、心外膜点状出血、肾脏质地变软如泥、脑严重水肿等。选取脑组织制备病理切片，在镜下可观察到大脑皮质中性粒细胞浸润及中性粒细胞性血管管套现象；深层脑组织进行触片染色后，在镜下可见单个或成对排列的革兰氏阳性球菌，并分离出1株可疑细菌，命名为NEF1；采用Mega 6.0生物软件依据该分离株的16S rRNA基因而绘制的遗传进化树分析结果显示，分离株NEF1与屎肠球菌的国内分离株（MT197247.1）和伊朗分离株（KM495939.1）聚类在同一分支上，而与GenBank中其他屎肠球菌参考菌株遗传距离较远；药物敏感性试验结果表明，分离株NEF1只对环丙沙星呈现中度敏感，而对青霉素等其他选定的抗生素类药物均呈现明显的耐药性。本试验从病死羊脑组织中成功分离并鉴定了1株屎肠球菌NEF1，同时通过药物敏感性试验方法筛选出来的敏感药物有效控制了该病在发病羊场的进一步蔓延，为该地区羊细菌性疾病的有效防治提供了行之有效的试验数据。
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| 关 键 词： | 羊 屎肠球菌 分离与鉴定 药物敏感性试验 防治 |
| 收稿时间： | 2021-04-22 |
Isolation and Identification of a Pathogenic Enterococcus faecium from Sheep |
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| BAI Weiqin,LI Hong,MENG Ke,ZI Zhanfei,LI Jie,ZHAO Lijun,WANG Jinling,GE Riletu,LI Qi,JI Lintai.Isolation and Identification of a Pathogenic Enterococcus faecium from Sheep[J].China Animal Husbandry & Veterinary Medicine,2021,48(10):3889-3895. |
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| Authors: | BAI Weiqin LI Hong MENG Ke ZI Zhanfei LI Jie ZHAO Lijun WANG Jinling GE Riletu LI Qi JI Lintai |
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| Institution: | 1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;2. Ejin Horo Hoshoh Animal Disease Prevention and Control Center, Ordos 017200, China |
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| Abstract: | The unexplained death of sheep occurred in a sheep farm in Ordos city. In order to find the cause of the disease and formulate treatment and prevention measures, this study confirmed the disease by clinical examination, laboratory pathology and molecular biology diagnosis methods, and provided treatment plan. In this experiment, the dead sheep were used as the main materials. Firstly, the clinical examination was carried out, including autopsy and histopathological sampling. Then, the brain tissue was taken back to the laboratory by routine aseptic method for contact film, staining and microscopic examination, and bacteria isolation. Autopsy of the dead sheep showed congestion and consolidation of lung, punctate hemorrhage of epicardium, soft and muddy texture of kidney, severe brain edema. Under the microscope, the infiltration of neutrophils in cerebral cortex and the phenomenon of neutrophil vascular cannula were observed. After contact staining in deep brain tissue, Gram positive cocci were found in single or paired arrangement under microscope, and a suspicious bacterium was isolated and named NEF1. Based on the 16S rRNA gene of the isolate, the phylogenetic tree analysis by Mega 6.0 software showed that the isolate NEF1 was clustered on the same branch with the domestic isolate (MT197247.1) and the Iranian isolate (KM495939.1) of Enterococcus faecium, but was far away from other reference strains of Enterococcus faecium in GenBank. The drug sensitivity test showed that NEF1 was only moderately sensitive to ciprofloxacin, but obviously resistant to penicillin and other selected antibiotics. A strain of Enterococcus faecium NEF1 was successfully isolated and identified from the brain tissue of dead sheep in this experiment. Meanwhile, the sensitive drugs screened by the drug sensitivity test method effectively controlled the further spread of the disease in the diseased sheep farms. It provided effective experimental data for effective prevention and control of sheep bacterial diseases in this area. |
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| Keywords: | sheep Enterococcus faecium isolation and identification drug sensitivity test prevention and treatment |
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