| 活禽产业链中大肠杆菌耐药性及耐药基因调查 |
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| 引用本文： | 袁敬知,杨莉瑶,陈思宇,蔡宗伶,李珣,廖玉英,王晓晔.活禽产业链中大肠杆菌耐药性及耐药基因调查[J].中国畜牧兽医,2021,48(7):2661-2670. |
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| 作者姓名： | 袁敬知 杨莉瑶 陈思宇 蔡宗伶 李珣 廖玉英 王晓晔 |
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| 作者单位： | 1. 广西大学动物科学技术学院, 南宁 530004;2. 广西壮族自治区畜牧研究所, 南宁 530001;3. 重庆市渝东卫生学校, 重庆 408300 |
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| 基金项目： | 崇左市科技计划项目（崇科FA2019006）；南宁市武鸣区科学研究与技术开发项目（20190101）；国家现代农业产业体系广西肉鸡产业创新团队建设专项（nycytxgxcxtd）；南宁市科学研究与技术开发计划项目（20202106） |
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| 摘 要： | 为了解家庭农场供货模式下"养殖-销售"活禽产业链中各环节大肠杆菌耐药性及耐药基因流行情况，本研究共采集132份来自自贡市沿滩区某一农贸市场及其活禽供货的33家家庭农场的样品，通过细菌分离纯化、形态学鉴定、特异性引物PCR检测、系统发育群PCR检测、药敏试验及耐药基因鉴定等方法对细菌特性进行分析。研究结果显示，83株分离株在伊红美蓝培养基上形成带有金属光泽的黑色菌落，镜检显示为革兰阴性短杆菌，且特异性引物检测结果均显示阳性，因此，确定83株分离株均为大肠杆菌。系统发育群鉴定结果发现，83株分离株系统发育群集中于A群（53.02%）及B1群（20.48%）；药敏试验结果显示，83株菌株均为多重耐药菌，其中硫酸黏杆菌素、四环素耐药率最高，均为100%，氨苄西林、头孢呋辛、氨曲南、氟苯尼考耐药率均超过90%；耐药基因结果显示，mcr-1、mcr-3、blaTEM、blaSHV、blaCTX-M-group 1、blaCTX-M-group 9、qnrS、aac（6'）-Ⅰb-cr、tetA和tetB等耐药基因均有检出，检出率均低于对应抗菌药的耐药率，符合预期结果。此外，统计结果显示，家庭农场细菌耐药率和耐药基因携带率低于农贸市场。本研究结果表明，农贸市场成为活禽产业链中耐药菌及耐药基因的集散地，可为指导禽大肠杆菌临床检测、诊断及合理用药提供试验依据，同时为评估活禽产业链耐药性传播风险提供可靠数据。
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| 关 键 词： | 活禽产业链 大肠杆菌 系统发育群 耐药性 耐药基因 |
| 收稿时间： | 2020-11-18 |
Investigation of Antimicrobial Resistance and Antimicrobial Resistance Genes of E.coli from the Live Poultry Production Chain |
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| YUAN Jingzhi,YANG Liyao,CHEN Siyu,CAI Zongling,LI Xun,LIAO Yuying,WANG Xiaoye.Investigation of Antimicrobial Resistance and Antimicrobial Resistance Genes of E.coli from the Live Poultry Production Chain[J].China Animal Husbandry & Veterinary Medicine,2021,48(7):2661-2670. |
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| Authors: | YUAN Jingzhi YANG Liyao CHEN Siyu CAI Zongling LI Xun LIAO Yuying WANG Xiaoye |
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| Institution: | 1. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;2. The Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Nangning 530001, China;3. Chongqing Yudong Health School, Chongqing 408300, China |
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| Abstract: | To understand the proportion of antimicrobial resistance and antimicrobial resistance genes of Escherichia coli from different links of the live poultry production chain in the family farm supply mode,in this study,132 samples were collected from one farmers' market and 33 family farms in Yantan town,Zigong city.Methods of bacteria isolation and purification,morphological examination,specific PCR detection,phylogenetic group detection,drug sensitivity test and antimicrobial resistance genes detection were used for the analysis of bacterial characteristics.The results showed that 83 isolates were E.coli strains which were black and metallic lustre colonies in EMB,Gram-negative bacteria under microscope and positive in specific primer test.Phylogenetic group detection results showed that 83 strains were concentrated in Group A (53.02%) and Group B1 (20.48%).The results of drug resistance test showed that 83 strains were multi-drug resistant bacteria,among which proportion drug resistance of colstin and tetracycline were 100% and proportion drug resistance of ampicillin,cefuroxime,aztreonam and fluorobenzene were over 90%.The results of antimicrobial resistance genes detection showed that antimicrobial resistance genes such as mcr-1, mcr-3, blaTEM, blaSHV, blaCTX-M-group 1, blaCTX-M-group 9, qnrS, aac(6')-Ⅰb-cr, tetA and tetB were detected and proportion of these genes were lower than proportion of antimicrobial resistance.In addition,the analysis results showed that proportion of antimicrobial resistance and antimicrobial resistance genes of E.coli from family farm was lower than that from farmers' market.This study proved that farmers' market had the aggregation function of antimicrobial resistance bacterial and antimicrobial resistance genes in live poultry production chain.This study provided experiment basis for the detection,diagnosis and treatment of avian Escherichia coli.As the same time,the study provided reliable data to assess transmission risk of antimicrobial resistant in live poultry production chain. |
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| Keywords: | live poultry production chain Escherichia coli phylogeny groups antimicrobial resistance antimicrobial resistance genes |
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