| 高效液相色谱荧光检测法检测鸡肉中甲砜霉素残留 |
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| 引用本文： | 谢恺舟,徐东,陈书琴,谢星,黄玉萍,贾龙飞,郭辉生,王金玉.高效液相色谱荧光检测法检测鸡肉中甲砜霉素残留[J].中国畜牧兽医,2011,38(3):163-166. |
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| 作者姓名： | 谢恺舟 徐东 陈书琴 谢星 黄玉萍 贾龙飞 郭辉生 王金玉 |
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| 作者单位： | （1.扬州大学动物科学与技术学院，江苏省动物遗传繁育与分子设计重点实验室，江苏扬州 225009； 2.扬州大学兽医学院，江苏扬州 225009) |
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| 基金项目： | 国家肉鸡产业技术体系资助，江苏省高新技术计划项目，江苏省科技成果转化专项 |
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| 摘 要： | 研究建立了用高效液相色谱荧光法检测鸡肉中甲砜霉素残留的方法。鸡肉经丙酮、二氯甲烷提取，饱和正己烷脱脂，氮吹仪吹干浓缩后，以乙腈―磷酸二氢钠溶液（0.01 mol/L，含0.005 mol/L十二烷基硫酸钠和0.1%三乙胺)（32∶68，V/V）为流动相，流速为1.0 mL/min，荧光检测激发波长为225 nm，发射波长为290 nm。甲砜霉素在0.01～1.75 mg/L浓度范围内，本方法线性关系良好，相关系数为0.9991。当甲砜霉素添加水平为5～500 μg/kg时，该方法平均回收率为79.49%～89.71%，相对标准偏差为4.96%～9.61%；日内相对标准偏差为5.68%～7.47%；日间相对标准偏差为8.89%～11.32%。甲砜霉素检测限为1.5 μg/kg（S/N＝3），定量限5 μg/kg（S/N＝10）。所建立的方法简便、快速、灵敏度高，适用于鸡肉中甲砜霉素残留的高灵敏度检测。
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| 关 键 词： | 高效液相色谱 荧光检测法 甲砜霉素 鸡肉 残留 |
Determination of Residues of Thiamphenicol in Chicken Muscle by HPLC with Fluorescence Detection |
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| XIE Kai-zhou,XU Dong,CHEN Shu-qin,XIE Xing,HUANG Yu-ping,JIA Long-fei,GUO Hui-sheng,WANG Jin-yu.Determination of Residues of Thiamphenicol in Chicken Muscle by HPLC with Fluorescence Detection[J].China Animal Husbandry & Veterinary Medicine,2011,38(3):163-166. |
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| Authors: | XIE Kai-zhou XU Dong CHEN Shu-qin XIE Xing HUANG Yu-ping JIA Long-fei GUO Hui-sheng WANG Jin-yu |
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| Institution: | (1.Key Laboratory for Animal Genetic，Breeding，Reproduction and Molecular Design of Jiangsu Province，College of Animal Science and Technology，Yangzhou University,Yangzhou 225009，China；2.College of Veterinary Medicine，Yangzhou University，Yangzhou 225009，China) |
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| Abstract: | A high performance liquid chromatography with fluorescence detection(HPLC-FLD) method was built for determination of residues of thiamphenicol(TAP) in chicken muscle. TAP was extracted with acetone and dichloromethane from chicken muscle. Extract solution was degreased with n-hexane and evaporated with nitrogen evaporator，the mobile phase was acetonitrile-sodium dihydrogen phosphate solution(0.01 mol/L，containing 0.005 mol/L SDS and 0.1% triethylamine)(32∶68)，the flow rate was 1.0 mL/min. The fluorescence detection was with an excitation wavelength of 225 nm and an emission wavelength of 290 nm. The data showed that the linear relationship of TAP was nice (r＝0.9991) in 0.01 mg/L to 1.75 mg/L concentration range. At spiked level 5 to 500 μg/kg for TAP，the average recovery ranged from 79.49% to 89.71%，relative standard deviation(RSD) ranged from 4.96% to 9.61%，the within-day RSD ranged from 5.68% to 7.47%，the between day RSD ranged from 8.89% to 11.32%. The lowest detection limit of TAP was 1.5 μg/kg(S/N＝3) and the lowest quantitative limit of TAP was 5 μg/kg (S/N＝10). This HPLC-FLD method is simple，rapid，high sensitivity and suitable for high sensitivity determination of TAP in chicken muscle. |
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| Keywords: | high performance liquid chromatography（HPLC） fluorescence detection thiamphenicol（TAP） chicken muscle residues |
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