| 猪繁殖与呼吸综合征病毒、猪圆环病毒2型与副猪嗜血杆菌混合感染的诊断及病原分析 |
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| 引用本文： | 邓同炜,薛亚南,卢建洲,夏艳勋,陈露露,郭懿文,彭丽明,蒋增海,乔宏兴,徐耀辉,张晓战.猪繁殖与呼吸综合征病毒、猪圆环病毒2型与副猪嗜血杆菌混合感染的诊断及病原分析[J].中国畜牧兽医,2020,47(10):3401-3409. |
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| 作者姓名： | 邓同炜 薛亚南 卢建洲 夏艳勋 陈露露 郭懿文 彭丽明 蒋增海 乔宏兴 徐耀辉 张晓战 |
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| 作者单位： | 1. 河南牧业经济学院动物医药学院, 郑州 450046;2. 河南省猪病防控工程技术研究中心, 郑州 450046;3. 河南省渑池县农业农村局, 三门峡 472400 |
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| 基金项目： | 河南牧业经济学院预防兽医重点学科项目（MXK2016102）；河南牧业经济学院博士启动基金项目（906/24030027、906/24030090） |
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| 摘 要： | 为确定河南开封某猪场发生猪呼吸系统疾病综合征（PRDC）的病原，本研究无菌采集病死保育猪肺脏、心脏和脾脏等组织样品，进行细菌学检验和药敏试验，通过PCR/RT-PCR检测样品中猪繁殖与呼吸综合征病毒（PRRSV）、猪圆环病毒2型（PCV2）、猪流感病毒（SIV）、猪伪狂犬病病毒（PRV）、猪瘟病毒（CSFV）和猪肺炎支原体（Mhp）等病原，并对核酸阳性病毒性病原的抗原结构基因进行测序和遗传演化分析。结果表明，通过细菌分离培养、形态观察、卫星现象观察和16S rRNA基因鉴定，从病死保育猪体内分离鉴定出1株副猪嗜血杆菌（Hps），药敏实验表明该菌株对对氨苄西林、阿莫西林克拉维酸、头孢噻呋和四环素几种药物敏感。核酸检测PRRSV和PCV2核酸阳性，分别命名为PRRSV/HN-2019和PCV2/HN11-2019；进一步对PRRSV/HN-2019和PCV2/HN11-2019的结构基因分析发现，PRRSV/HN-2019与与NADC30分支的毒株亲缘关系较近，属于NADC30-like毒株；PCV2/HN11-2019与PCV-2d分支的毒株亲缘关系较近，属于PCV-2d分支。综上所述，本研究确定该猪场存在PRRSV、PCV2和Hps的混合感染，为该猪场下一步的PRDC有效防控提供了参考依据。
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| 关 键 词： | 猪呼吸系统疾病综合征(PRDC) 副猪嗜血杆菌(Hps) 猪繁殖与呼吸综合征病毒(PRRSV) 猪圆环病毒2型(PCV2) 混合感染 病原分析 |
| 收稿时间： | 2020-04-02 |
Diagnosis and Pathogen Analysis of Mixed Infection of Porcine Reproductive and Respiratory Syndrome Virus,Porcine Circovirus Type 2 and Haemophilus parasuis |
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| DENG Tongwei,XUE Yanan,LU Jianzhou,XIA Yanxun,CHEN Lulu,GUO Yiwen,PENG Liming,JIANG Zenghai,QIAO Hongxing,XU Yaohui,ZHANG Xiaozhan.Diagnosis and Pathogen Analysis of Mixed Infection of Porcine Reproductive and Respiratory Syndrome Virus,Porcine Circovirus Type 2 and Haemophilus parasuis[J].China Animal Husbandry & Veterinary Medicine,2020,47(10):3401-3409. |
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| Authors: | DENG Tongwei XUE Yanan LU Jianzhou XIA Yanxun CHEN Lulu GUO Yiwen PENG Liming JIANG Zenghai QIAO Hongxing XU Yaohui ZHANG Xiaozhan |
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| Institution: | 1. College of Veterinary Medicine, Henan University of Animal Husbandry of Economy, Zhengzhou 450046, China;2. Henan Engineering and Technology Research Center of Veterinary Biotechnology, Zhengzhou 450046, China;3. Mianchi Agricultural and Rural Burea in Henan Province, Sanmenxia 472400, China |
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| Abstract: | To identify the causative agent of porcine respiratory disease complex (PRDC) occurred at a pig farm in Kaifeng city,Henan province,we identified the potential causative bacteria of the morbid nursing piglets by bacterial test and drug sensitivity test of the clinical samples (lung,liver and spleen),and detected the common potential pathogens causing PRDC diseases by PCR and RT-PCR assays,including porcine reproductive and respiratory syndrome virus (PRRSV),porcine circovirus type 2 (PCV2),swine influenza virus (SIV),pseudorabies virus (PRV),classical swine fever virus (CSFV) and Mycoplasma hyopneumoniae (Mhp),and then sequcing and phylogenetic analysis of the structural gene of positive causative pathogens were carried out.The results showed that a Haemophilus parasuis (Hps) strain were isolated and identified by the observation of bacterial morphology and satellite phenomenon,and the sequence analysis of 16S rRNA gene.The drug sensitivity test showed that the Hps strain was sensitive to ampicillin,amoxicillin-clavulanic acid,ceftiofur and tetracycline.Meanwhile,PCR and RT-PCR assays indicated that all the samples were positive for PRRSV and PCV2,and named the involved strain as PRRSV/HN-2019 and PCV2/HN11-2019,respectively.Sequencing and phylogenetic analysis of the ORF5 gene of the newly identified PRRSV revealed that the PRRSV/HN-2019 strain was closely related to the NADC30-like strains and grouped into NADC30-like genotype clade.And cap gene of the newly identified PCV2 strain the PCV2/HN11-2019 strain was closely related to the PCV-2d strains and grouped into PCV-2d genotype clade.In conclusion,this study demonstrated that the morbid piglets were co-infected with PRRSV,PCV2 and Hps,which provided a basis for the development of effective control strategies in the pig farm. |
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| Keywords: | porcine respiratory disease complex (PRDC) Haemophilus parasuis (Hps) porcine reproductive and respiratory syndrome virus (PRRSV) porcine circovirus type 2 (PCV2) co-infection etiology analysis |
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