Effect of Human Menopausal Gonadotropin,17β-estradiol and Epidermal Growth Factor on the Quality of in vitro Maturation Bovine Oocytes |
| |
| Authors: | TANG Shuang AN Wen-zhong JIANG Xin-long LI Xiang-chen |
| |
| Institution: | 1. Liaoning Provincial Key Laboratory for Agricultural Biotechnology, College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China;2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
| |
| Abstract: | For optimizing in vitro maturation system of bovine oocytes,we firstly examined the influence of four different hormonal regimes(FSH+LH,HMG,FSH+LH+E2 and HMG+E2) on oocyte maturation rates.Then we studied the effects of epidermal growth factor (EGF) in the above defined medium on bovine oocyte maturation,in vitro development and quality of parthenogenetic embryos.The cell apoptotic index of parthenogenetic blastocysts was detected by TUNEL.No significant difference was observed in maturation rates in four groups supplemented with different hormones.However,human menopausal gonadotropin (HMG) provided steady maturation results in replicates.Maturation of oocytes was promoted by supplementation with 17β-estradiol (E2).Combination of HMG and E2 gave rise to steady and efficient mature results.The presence of EGF at 30 ng/mL concentration significantly increased maturation rate and blastocyst rate and reduced apoptotic cells in parthenogenetic blastocysts.Therefore,the optimal oocyte maturation solution could be supplemented with 0.075 IU/mL HMG,1 μg/mL E2 and 30 ng/mL EGF. |
| |
| Keywords: | bovine oocytes epidermal growth factor human menopausal gonadotropin in vitro maturation |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
