Construction and High Level Expression of Prokaryotic Expression Vector of Pig Interferon-δ Gene |
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| Authors: | LI Hong LIU Cheng-qian YAN Hua-xiang WANG Jia-hao YI Jian-zhong |
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| Institution: | 1. Institute of Animal Husbandry & Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;2. Shanghai Vocational College of Agriculture and Forestry, Shanghai 201699, China |
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| Abstract: | Interferon (IFN) had recieved much more attention because of its broad-spectrum antiviral, antitumor activity and immune regulation.One pair of primers were designed for amplifying pig IFN-δ gene with PCR method according to the relevant sequence from GenBank.The IFN-δ gene was cloned into prokaryotic expression vector, and the recombinant plasmid was obtained.We transformed the recombinant plasmid into E.coli Transetta BL21(DE3) strain and the protein expression was identified by SDS-PAGE and Western blotting.The results showed that pET-30a-DsbA-IFN-δ recombinant protein was 20 ku and expressed mainly in the form of inclusion body, these expressed protein could specific react with His-tag monoclonal antibody. |
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| Keywords: | pig interferon-δ prokaryotic expression |
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