Development and Application of Sandwich ELISA for Diagnosis of Fascioliasis gigantica in Early Stage |
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| Authors: | LI Xue WEI Zhi-peng ZHAO Ming-long XU Xin-ting LIU Yu WU Wen-de JIANG Wei HUANG Tian LONG Fei-xiang CHEN Han-zhong |
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| Institution: | College of Animal Science and Technology, Guangxi University, Nanning 530004, China |
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| Abstract: | To establish a method for the diagnosis of Fascioliasis gigantica in early stage, five hybridoma cell lines were recovered and used for preparation of monoclonal antibodies.7D2 was used as a capture antibody and 7D1 as detection antibody.Coating antibody dilution was 1:6 400 (0.208 μg/mL), 5% nonfat milk was used as blocking solution and detection antibody dilution was 1:10 000 (0.200 μg/mL).The detection limit of the sandwich ELISA was 1:3 200 (0.156 μg/mL), with good specificity and stability, and there was no cross reaction with other kinds of parasite antigen.The results showed that the method provided the important conditions and theoretical basis for the diagnosis of Fascioliasis gigantica in early stage. This would save unnecessary economic losses to the livestock, and it had clinical application value. |
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| Keywords: | early diagnosis monoclonal antibody Fascioliasis gigantica sandwich ELISA |
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