| 一例肉鸡大肠杆菌和粪肠球菌混合感染的诊治 |
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| 引用本文： | 管夕柱,李开锋,张启迪,刘文华.一例肉鸡大肠杆菌和粪肠球菌混合感染的诊治[J].中国畜牧兽医,2012,39(8):223-226. |
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| 作者姓名： | 管夕柱 李开锋 张启迪 刘文华 |
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| 作者单位： | 1. 青岛农业大学动物科技学院,山东青岛,266109
2. 威海出入境检验检疫局,山东威海,264200 |
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| 基金项目： | 山东出入境检验检疫局科技项目 |
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| 摘 要： | 本试验对某发病肉鸡场的病死鸡进行了病原学诊断。通过病原分离、生化试验、动物试验、血清型鉴定、PCR扩增等方法对分离细菌进行了鉴定,最终确定为大肠杆菌和粪肠球菌的混合感染。在此基础上进行了药敏试验,筛选出了大肠杆菌的高敏药物阿米卡星和庆大霉素,而粪肠球菌只对某些氟喹诺酮类药物中度敏感,其余均显示为耐药。将阿米卡星、庆大霉素和恩诺沙星用于发病鸡群的治疗,很快控制了疫情,及时减少了经济损失。
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| 关 键 词： | 肉鸡 大肠杆菌 粪肠球菌 PCR 药敏试验 |
| 收稿时间： | 2012-01-07 |
Diagnosis and Treatment of the Mixed Infection of E.coli and Enterococcus faecalis of Broiler |
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| GUAN Xi-zhu
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LI Kai-feng
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ZHANG Qi-di
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LIU Wen-hua.Diagnosis and Treatment of the Mixed Infection of E.coli and Enterococcus faecalis of Broiler[J].China Animal Husbandry & Veterinary Medicine,2012,39(8):223-226. |
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| Authors: | GUAN Xi-zhu LI Kai-feng ZHANG Qi-di LIU Wen-hua |
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| Institution: | 1. College of Animal Science and Veterinary Medicine, Qingdao Agricultural University,Qingdao 266109,China;
2. Weihai Entry-exit Inspection and Quarantine Bureau,Weihai 264200,China |
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| Abstract: | Etiological diagnosis were done to the dead broiler from one farm which experienced the epidemic in this experiment. The isolated bacteria were identified by methods of pathogenic separation, biochemical tests, animal experiments, serotype identification, PCR amplification and so on. As a result, the mixed infections of E.coli and Enterococcus faecalis were identified. Further more, drug susceptibility tests were taken according to the isolated bacteria and the high sensitivity antibiotics against E.coli named amikacin and gentamycin were selected, while only some fluoroquinolones showed moderately sensitive against Enterococcus faecalis among the 21 antibiotics. Then amikacin, gentamycin and enrofloxacin were used for the treatment of broiler, which showed quick effects on controlling the death, and greatly reduced the economic losses caused by the epidemic. |
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| Keywords: | broiler E coli Enterococcus faecalis PCR drug susceptibility test |
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