| 犬瘟热病毒上海分离株M蛋白编码基因的克隆与序列分析 |
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| 引用本文： | 张永霞,王金福,张似青.犬瘟热病毒上海分离株M蛋白编码基因的克隆与序列分析[J].中国畜牧兽医,2008,35(12):73-75. |
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| 作者姓名： | 张永霞 王金福 张似青 |
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| 作者单位： | 上海农林职业技术学院, 上海 201600 |
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| 基金项目： | 上海农林职业技术学院科研资助项目 |
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| 摘 要： | 本研究以犬瘟热病毒(canine distemper virus，CDV)上海分离株的总RNA为模板,根据GenBank中已报道的CDV M蛋白基因序列，设计合成1对特异性引物，RT PCR扩增M蛋白基因，并进行克隆与序列分析。结果显示，CDV上海分离株M蛋白基因序列与CDV A75/17 株、Onderstepoort疫苗株等其它7株的同源性在95%以上；编码氨基酸的同源性也高于97%。推测M蛋白是一个保守性非常强的结构蛋白。
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| 关 键 词： | 犬瘟热病毒 M蛋白 序列分析 |
The Cloning and Sequence Analysis of M Protein Coding Gene of SH Strain of Canine Distemper Virus |
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| ZHANG Yong-xia,WANG Jin-fu,ZHANG Si-qing.The Cloning and Sequence Analysis of M Protein Coding Gene of SH Strain of Canine Distemper Virus[J].China Animal Husbandry & Veterinary Medicine,2008,35(12):73-75. |
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| Authors: | ZHANG Yong-xia WANG Jin-fu ZHANG Si-qing |
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| Institution: | Shanghai Vocational Technical College of Agriculture & Forestry,Shanghai 201600,China |
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| Abstract: | According to the reported gene sequence of M protein of canine distemper virus(CDV) in GenBank,a special pair of primers were designed to RT-PCR amplify M protein gene by using the total RNA of CDV SH stain as a template.The results showed that the homology of gene sequence between CDV SH strain and A75/17,Onderstepoort stain and other seven strain were above 95%;the homology of coding amino acid between CDV SH strain and the others were above 97%.From this information,it can be predicted that M protein has highly conservative property. |
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| Keywords: | canine distemper virus M protein sequence analysis |
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