| 向海地区绿头鸭体内1膜壳属(Hymenolepis spp.)绦虫的分离鉴定 |
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| 作者姓名： | 刘莹 李杰 李秀云 王丽新 翟琪安 沈冬冬 彭智伟 侯志军 |
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| 作者单位： | 东北林业大学野生动物资源学院;陕西佛坪国家级自然保护区管理局;哈尔滨北方森林动物园 |
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| 基金项目： | 国家重点研发计划项目(2016YFC1201600);中央高校基本科研业务费专项资金(2572018BE07);国家林业局疫源疫病监测项目 |
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| 摘 要： | 为了对绿头鸭肠道内分离的绦虫进行种属鉴定,本试验对向海地区的10只绿头鸭小肠内分离出的3条绦虫进行了形态学和分子生物学的鉴定。经形态学观察测量发现,该绦虫为小型绦虫,全长35 mm,头节呈球形,具有4个吸盘和1个短而圆、可自由伸缩的顶突。顶突上有20～30个小钩,排成一圈。成节有3个圆球形的睾丸,横列在节片中部,储精囊较发达,符合膜壳属绦虫的形态学特征。扩增cox1基因序列并进行序列建树分析,结果支持该绦虫为膜壳属绦虫。综合该虫的形态学特征及分子生物学分析,确定该绦虫为膜壳属绦虫。
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| 关 键 词： | 膜壳属 绦虫 绿头鸭 |
Isolation and Identificationof a Hymenolepis spp. From Infected Mallard( Anas platyrhynchos) in the Xianghai Area,China |
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| Authors: | LIU Ying LI Jie LI Xiuyun WANG Lixin ZHAI Qian SHEN Dongdong PENG Zhiwei HOU Zhijun |
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| Institution: | (College of Wildlife Resources,Northeast ForestryUniversity,Harbin,150040,China;National Nature Reserve Management Bureau in Shanxi Foping,Foping,723400,China;Harbin Northern Forest Zoo,Harbin,150332,China) |
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| Abstract: | In order to identify the species of tapeworm isolated from the intestines of mallard,three tapeworms isolated from the small intestine of 10 mallard ducks in the Xianghai area were identified by morphology and molecular biology methods.Morphological characters were:small body size of 35 mm length;a spherical scolex;4 sucking plates;and a short,round and freely telescopic rostellum.On the rostellum,20-30 hooks were arranged in a circle.Three spherical testes occurred in a horizontal line in the middle of mature proglottids,and the seminal vesicles were relatively developed.These characters were identical to those of Hymenolepis spp.In addition,the cox 1 gene sequence was amplified to perform sequence alignment to construct a phylogenetic tree.This confirmed that tapeworm was a species of Hymenolepis.Hence,the tapeworm was identified as Hymenolepis sp.based on morphological and molecular characteristics. |
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| Keywords: | Hymenolepis spp Tapeworm Anas platyrhynchos |
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