| 几种禾本科牧草内生细菌的分布特性 |
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| 引用本文： | 车建美,刘波,张彦,胡桂萍,黄勤楼,陈忠钿,翁伯琦.几种禾本科牧草内生细菌的分布特性[J].草业学报,2010,19(3):124-131. |
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| 作者姓名： | 车建美 刘波 张彦 胡桂萍 黄勤楼 陈忠钿 翁伯琦 |
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| 作者单位： | 1.福建省农业科学院农业生物资源研究所,福建 福州350003;2.福建省农业科学院农业生态研究所,福建 福州 350013 |
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| 基金项目： | "十一五"国家科技支撑计划，福建省财政专项-福建省农业科学院科技创新团队建设基金 |
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| 摘 要： | 不同禾本科牧草植株内生细菌含量差异显著,杂交狼尾草闽牧六号内生细菌含量最高,达26.800×104CFU/g。具体到各个部位,禾本科牧草不同部位内生细菌含量差异也显著。通过微生物鉴定系统对内生菌进行鉴定的结果表明,64株内生细菌中与MIDI数据库比较相似性指数大于0.500,可确定其菌种名称的有50株,属于19个种,隶属于12个属。以分离到的19种内生菌为样本,以各菌株在各部位出现与否为指标,用类平均法对数据进行系统聚类分析,当λ=8.35时,可将19种内生细菌分为4个大类群。从聚类结果显示,内生菌与宿主植株和不同分离部位都存在相关性,与宿主植株的关系更密切。
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| 关 键 词： | 禾本科牧草 内生细菌 脂肪酸检测 分布特性 |
| 收稿时间： | 1900-01-01; |
Distribution characteristics of endophytic bacteria isolated from forage grasses |
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| CHE Jian-mei,LIU Bo,ZHANG Yan,HU Gui-ping,HUANG Qin-lou,CHEN Zhong-dian,WENG Bo-qi.Distribution characteristics of endophytic bacteria isolated from forage grasses[J].Acta Prataculturae Sinica,2010,19(3):124-131. |
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| Authors: | CHE Jian-mei LIU Bo ZHANG Yan HU Gui-ping HUANG Qin-lou CHEN Zhong-dian WENG Bo-qi |
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| Institution: | 1.Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China;2.Research Center of Ecological Agriculture, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China |
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| Abstract: | The endophytic bacteria were isolated from different forage grasses according to culture method and identified by using fatty-acid identification technology. The results showed that the contents of endophytic bacteria of hybrid pennisetum “Minmu six”, which was 26.800×104 CFU/g, was higher than other forage grasses. The endophytic bacteria isolated from different forage grasses were from 19 species belonging to 12 genera. The cluster analysis results showed that the 19 strains could be separated into 4 groups. It was concluded that the distribution characterics of endophytic baceria were related to different forage grasses and different isolated parts, especially to the different forage grasses. |
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| Keywords: | forage grasses endophytic bacteria fatty-acid detection distribution characteristics |
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