| 一株抗马铃薯坏疽病莫海威芽孢杆菌(Bacillus mojavensis ZA1)培养条件的优化 |
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| 引用本文： | 冯中红,畅涛,杨成德,薛莉,李旭,杨小利.一株抗马铃薯坏疽病莫海威芽孢杆菌(Bacillus mojavensis ZA1)培养条件的优化[J].草业学报,2016,25(2):77-86. |
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| 作者姓名： | 冯中红 畅涛 杨成德 薛莉 李旭 杨小利 |
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| 作者单位： | 草业生态系统教育部重点实验室，甘肃农业大学草业学院，甘肃省草业工程实验室，中-美草地畜牧业可持续发展研究中心，甘肃 兰州 730070 |
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| 基金项目： | 国家自然科学基金(No．31160122)资助。 |
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| 摘 要： | 为了提高生防菌株莫海威芽孢杆菌(Bacillus mojavensis ZA1)液体发酵的生物量,利用单因素试验设计与响应曲面试验设计相结合的方法对ZA1摇瓶发酵条件进行了优化。结果表明,ZA1的最佳培养基配比为氯化铵14.25g、玉米粉19g、马铃薯237g、水1000mL,最佳发酵条件为pH 7.7、培养温度28℃、转速180r/min及发酵时间36h,ZA1优化后活菌数为4.12×1010 CFU/mL。通过中心组合试验设计确定ZA1在10L发酵罐中的最佳溶氧量为60%和转速为180r/min;最优条件下,发酵ZA1的放罐时间确定为36h,活菌数达到1.59×1011 CFU/mL。该结果为利用ZA1开发生防制剂奠定了基础。
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| 关 键 词： | 莫海威芽孢杆菌 发酵工艺 优化 |
| 收稿时间： | 2015-03-17 |
Optimization of culture conditions of a biocontrol agent Bacillus mojavensis ZA1 to antagonize Phoma foveata |
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| FENG Zhong-Hong,CHANG Tao,YANG Cheng-De,XUE Li,LI Xu,YANG Xiao-Li.Optimization of culture conditions of a biocontrol agent Bacillus mojavensis ZA1 to antagonize Phoma foveata[J].Acta Prataculturae Sinica,2016,25(2):77-86. |
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| Authors: | FENG Zhong-Hong CHANG Tao YANG Cheng-De XUE Li LI Xu YANG Xiao-Li |
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| Abstract: | This work aimed to identify optimum fermentation shaker-flask culture conditions for Bacillus mo-javensis strain ZA1.Various single culture factors were combined according to a Box-Behnken design.The op-timum ratio of ingredients for the culture medium for ZA1was found to be:14.25 g ammonium chloride,19 g corn flour,and 237 g potato,in 1000 mL distilled water.The optimum cultivation conditions were pH 7.7, temperature 28℃,and shaken at 180 r/min for 36 h.Under these conditions,the viable count of B .mojavens-is strain ZA1 was 4.12×10 10 colony-forming units per mL (CFU/mL).Further testing using the central com-posite design method in a 10 L fermentation tank found the optimum for dissolved oxygen level and shaker rota-tion speed to be 60% saturation and 180 r/min respectively.Under these conditions,when fermentation time was 36 h in the fermentation tank,the viable count of B .mojavensis strain ZA1 reached 1.59×10 11 CFU/mL. The results of this study lay a solid foundation for the development of methodology for large scale production of biocontrol agents. |
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| Keywords: | Bacillus mojavensis fermentation process optimization |
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