| 致病性猪源肠球菌16S rDNA-RFLP研究及系统进化分析 |
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| 引用本文： | 杨霞,陈陆,许瑞,常洪涛,刘红英,王川庆.致病性猪源肠球菌16S rDNA-RFLP研究及系统进化分析[J].中国兽医学报,2010,30(3). |
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| 作者姓名： | 杨霞 陈陆 许瑞 常洪涛 刘红英 王川庆 |
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| 作者单位： | 河南农业大学,牧医工程学院,河南,郑州,450002 |
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| 基金项目： | 河南省科技攻关资助项目(0624030030) |
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| 摘 要： | 为探讨分离自河南滑县、武陟等12个地区的猪源肠球菌的亲缘关系,采用对其16SrDNA基因的RFLP分析、序列分析及系统进化树的构建等方法,对12株疑似猪源肠球菌进行了研究;结果显示,12株分离株均与肠球菌同源性最高,同源率达98.1%～100%;而与猪链球菌同源率最高达85.5%,且进化树中12株菌株均位居与猪链球菌并列的肠球菌主干分支中。HandⅢ、XbaⅠ、EcoRⅠ分别对12株球菌的16SrDNA-RFLP分析发现,它们在该片段上有限制性位点,但没有产生限制性片段长度多态性;而HinfⅠ单酶切结果出现了限制性片段长度多态性,且12株分离株可分为两大rDNA图谱类群,与实际测序分析的酶切位点相一致,且找到鉴定肠球菌的350bp的分子标记。说明所分离12株球菌均是肠球菌,且不同地理种群已出现分化。
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| 关 键 词： | 致病性肠球菌 RFLP 同源性 进化树 |
The RFLP investigation and phylogenetic analysis of 16S rDNA of Enterococcus of swine |
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| YANG Xia,CHEN Lu,XU Rui,CHANG Hong-tao,LIU Hong-ying,WANG Chuan-qing.The RFLP investigation and phylogenetic analysis of 16S rDNA of Enterococcus of swine[J].Chinese Journal of Veterinary Science,2010,30(3). |
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| Authors: | YANG Xia CHEN Lu XU Rui CHANG Hong-tao LIU Hong-ying WANG Chuan-qing |
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| Institution: | YANG Xia,CHEN Lu,XU Rui,CHANG Hong-tao,LIU Hong-ying,WANG Chuan-qing (Engineering College of Animal Husb,ry , Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | By the methods of RFLP analysis of 16S rDNA,sequence analysis,the construction of the phylogenetic tree,relationship of the suspect strains of Enterococcus from swine in twelve regions isolated from Huaxian,Wuzhi and etc were investigated.The results showed:All the isolated strains shared 98.1% to 100% sequence homology with Enterococcus and 85.5% homology with the Streptococcus of swine;In the phylogenetic tree,the twelve isolated strains were in the main stem of Enterococcus.In addition,the isolated strai... |
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| Keywords: | Enterococcus RFLP homology phylogenetic tree |
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