| 高能低蛋白饲粮对蛋鸡肝脏apo B-100基因mRNA表达的影响 |
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| 引用本文： | 曹华斌,郭小权,张彩英,李浩棠,王小莺,罗军荣,胡国良.高能低蛋白饲粮对蛋鸡肝脏apo B-100基因mRNA表达的影响[J].中国兽医学报,2010,30(3). |
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| 作者姓名： | 曹华斌 郭小权 张彩英 李浩棠 王小莺 罗军荣 胡国良 |
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| 作者单位： | 江西农业大学,动物科学技术学院,江西,南昌,330045 |
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| 基金项目： | 国家自然科学基金资助项目(30160064;30760180) |
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| 摘 要： | 以海蓝褐蛋鸡为试验对象,探讨高能低蛋白日粮对蛋鸡肝脏载脂蛋白B-100mRNA表达关系的影响。选300日龄海蓝褐蛋鸡60只,随机分为2组,每组30只,分别饲喂正常饲粮(对照组,代谢能:2.75MJ/kg,粗蛋白:17%)、高能低蛋白饲粮(试验组,代谢能:2.99MJ/kg,粗蛋白:12.9%)。于试验0、30、60d每组选取体质量相近的试验蛋鸡6只,取肝脏样品,应用半定量逆转录聚合酶链式反应技术测定不同时期apoB-100mRNA在肝脏中的表达水平;结果显示:试验组载脂蛋白B-100mRNA表达在试验60d显著低于对照组(0.01P≤0.05)。说明高能低蛋白日粮能导致蛋鸡肝脏载脂蛋白B-100mRNA表达水平下降。
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| 关 键 词： | 蛋鸡 肝脏 高能低蛋白饲粮 载脂蛋白B-100 mRNA |
Effect of high energy-low protein diet on expression of apolipoprotein B-100 mRNA in liver of laying hens |
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| CAO Hua-bin,GUO Xiao-quan,ZHANG Cai-ying,LI Hao-tang,WANG Xiao-ying,LUO Jun-rong,HU Guo-liang.Effect of high energy-low protein diet on expression of apolipoprotein B-100 mRNA in liver of laying hens[J].Chinese Journal of Veterinary Science,2010,30(3). |
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| Authors: | CAO Hua-bin GUO Xiao-quan ZHANG Cai-ying LI Hao-tang WANG Xiao-ying LUO Jun-rong HU Guo-liang |
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| Institution: | CAO Hua-bin,GUO Xiao-quan,ZHANG Cai-ying,LI Hao-tang,WANG Xiao-ying,LUO Jun-rong,HU Guo-liang (College of Animal Science , Technology,Jiangxi Agricultural University,Nanchang 330045,China) |
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| Abstract: | The experiment was carried out to investigate the effect of high energy-low protein diet on expression of apolipoprotein B-100 mRNA in liver of laying hens.Sixty 300-day-age laying hens were stratified by weight and randomly assigned to two dietary treatments of 30 each,which were control group (AME:2.75 MJ/kg,CP:17%) and experimental group (AME:2.99 MJ/kg,CP:12.9%),respectively.All of the experimental laying hens were fed with corn-soybean meal for 60 days;the apolipoprotein B-100 mRNA expression level in ... |
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| Keywords: | laying hens liver high energy low protein diet apo B-100 mRNA |
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