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家鸡(Gallus domesticus)和鹌鹑(Coturnix coturnix)染色体核型及G带比较分析
引用本文:徐琪,陈国宏,张学余,王偕根,嵇宝华,李碧春,吴信生.家鸡(Gallus domesticus)和鹌鹑(Coturnix coturnix)染色体核型及G带比较分析[J].中国兽医学报,2005,25(5):547-549.
作者姓名:徐琪  陈国宏  张学余  王偕根  嵇宝华  李碧春  吴信生
作者单位:1. 扬州大学,动物科学与技术学院,江苏,扬州,225009
2. 中国农业科学院,家禽研究所,江苏,扬州,225003
3. 江苏省姜堰市农林局,江苏,姜堰,225500
基金项目:国家高新技术发展计划项目(863-2001AA243082),国家自然科学基金资助项目(30170673)
摘    要:采用外周血淋巴细胞培养法和胰酶处理法,对家鸡和鹌鹑染色体的核型和G带进行了研究。结果表明,家鸡和鹌鹑染色体数目均为2n=78,但染色体的形态存在明显差异,主要表现在NO.1、NO.4、NO.6、NO.8和Z染色体上。NO.1染色体,家鸡为m型,而鹌鹑为sm型;NO.4、NO.6、NO.8和Z染色体,家鸡分别为sm、sm、m、m型,而鹌鹑全为t型。G带分析结果显示,家鸡和鹌鹑的G带差异主要反映在NO.1、NO.2染色体上,这可能是由于臂间倒位所致。

关 键 词:家鸡  鹌鹑  染色体  核型  G带
文章编号:1005-4545(2005)05-0547-03
修稿时间:2004年3月1日

Comparison of the Karyotypes and G-banding Patterns between Domestic Fowl (Gallus domesticus) and Quail (Coturnix coturnix)
XU Qi,CHEN Guo-hong,ZHANG Xue-yu,WANG Xie-gen,JI Bao-hua,LI Bi-chun,WU Xin-sheng.Comparison of the Karyotypes and G-banding Patterns between Domestic Fowl (Gallus domesticus) and Quail (Coturnix coturnix)[J].Chinese Journal of Veterinary Science,2005,25(5):547-549.
Authors:XU Qi  CHEN Guo-hong  ZHANG Xue-yu  WANG Xie-gen  JI Bao-hua  LI Bi-chun  WU Xin-sheng
Institution:XU Qi~1,CHEN Guo-hong~
Abstract:Karyotype analysis was carried out on domestic fowls and quails using the peripheral lymphocyte culture techniques and G-banding patterns were obtained with trypsin and Giemsa staining.The results showed that the domestic fowl and quail had a diploid number of 78.But their positions of centromere of macrochromosomes were different from each other.NO.1 chromosome was metacentric,while that of quail was submetacentric.Besides,NO.4,6,8,Z chromosome of domestic fowl were submetacentric,submetacentric,metacentric,metacentric,repectively,whereas those of quail were all telocentric.Additionally,analysis of G-banding patterns for macrochromosomes of them indicated that there was great difference between NO.1 and NO.2 chromosome,which can be explained by the pericentric inversions.
Keywords:domestic fowl  quail  chromosome  karyotype  G-banding pattern
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