| 副猪嗜血杆菌ERIC-PCR指纹图谱多样性研究 |
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| 引用本文： | 贾爱卿,李春玲,王贵平,杨冬霞.副猪嗜血杆菌ERIC-PCR指纹图谱多样性研究[J].中国兽医学报,2010,30(3). |
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| 作者姓名： | 贾爱卿 李春玲 王贵平 杨冬霞 |
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| 作者单位： | 广东省农业科学院,兽医研究所,广东,广州,510640;广东省兽医公共卫生公共实验室,广东,广州,510640 |
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| 基金项目： | 广东省农业攻关项目(2007A020300005-11) |
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| 摘 要： | 建立了副猪嗜血杆菌ERIC-PCR分型技术,并应用于临床分离菌株的流行病学调查。试验结果表明,47株副猪嗜血杆菌产生28种不同的指纹图谱,分离自同一猪场和相同血清型的菌株表现出不同的指纹图谱,不能进行血清分型的副猪嗜血杆菌应用该方法可得到充分区分,证实副猪嗜血杆菌ERIC指纹图谱存在丰富的多样性,ERIC-PCR方法是副猪嗜血杆菌流行病学规律分析的有效方法。
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| 关 键 词： | 副猪嗜血杆菌 ERIC-PCR 指纹图谱 |
Characterization of the diversity of Haemophilus parasuis field isolates by use of ERIC-PCR |
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| JIA Ai-qing,LI Chun-ling,WANG Gui-ping,YANG Dong-xia.Characterization of the diversity of Haemophilus parasuis field isolates by use of ERIC-PCR[J].Chinese Journal of Veterinary Science,2010,30(3). |
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| Authors: | JIA Ai-qing LI Chun-ling WANG Gui-ping YANG Dong-xia |
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| Institution: | JIA Ai-qing1,2,LI Chun-ling1,WANG Gui-ping1,YANG Dong-xia1,2 (1.Institute of Veterinary Medicine,Guangzhou Academy of Agricultural Sciences,Guangzhou 510640,China,2.Guangzhou Key Laboratory of Veterinary Public Health,China) |
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| Abstract: | To establish the ERIC-PCR(Enterobacterial repetitive intergenic consensus-PCR)DNA fingerprinting technique for typing Haemophilus parasuis and apply it the epidemiological investigation.The result of polymorphism analysis could differentiate the 47 isolates into 28 groups,and the isolates from same farm or of same serotyping showed different genotype.And the isolates wich could not be serotyping could be genotyping by ERIC-PCR.The prevalent isolates gave difference ERIC-PCR fingerprint.ERIC-PCR DNA fingerpr... |
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| Keywords: | Haemophilus parasuis ERIC-PCR DNA fingerprint |
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