| 表达FAdV-8b Fiber蛋白重组FAdV-4的构建与鉴定 |
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| 引用本文： | 王白玉,黄庆,乔麒龙,杨盼盼,李永涛,王增,杨霞,苗玉和,赵军.表达FAdV-8b Fiber蛋白重组FAdV-4的构建与鉴定[J].中国兽医学报,2022(2):230-236. |
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| 作者姓名： | 王白玉 黄庆 乔麒龙 杨盼盼 李永涛 王增 杨霞 苗玉和 赵军 |
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| 作者单位： | ;1.河南农业大学动物医学院;2.福建圣维生物技术有限公司 |
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| 基金项目： | 国家自然科学基金资助项目(31772771)。 |
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| 摘 要： | 为了构建能够同时防控鸡肝炎-心包积液综合征(HHS)和包涵体肝炎(IBH)的新型疫苗,本研究利用禽腺病毒4型(FAdV-4)中国流行株CH/HNJZ/2015的反向遗传操作技术,将禽腺病毒8b型(FAdV-8b)中国流行株的全长Fiber基因插入至FAdV-4基因组中1 966 bp自然缺失区,构建感染性克隆并拯救出重组病毒rHNJZ-Fiber/FAdV8b,利用PCR、Western blot和间接免疫荧光试验对重组病毒rHNJZ-Fiber/FAdV8b进行鉴定。结果表明,FAdV-8b的Fiber基因得以准确插入,Fiber蛋白正确并稳定表达,重组病毒rHNJZ-Fiber/FAdV8b具有良好的遗传稳定性,在细胞中具有与亲本毒株相似的高滴度生长特性和复制动态。本研究构建的表达FAdV-8b中国流行株Fiber基因的重组FAdV-4将为同时研发HHS和IBH的有效疫苗提供新的思路。
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| 关 键 词： | 禽腺病毒4型 禽腺病毒8b型 Fiber蛋白 肝炎-心包积液综合征 包涵体肝炎 新型疫苗 |
Construction and identification of recombinant fowl adenovirus 4 expressing the fiber gene of fowl adenovirus 8b |
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| WANG Baiyu,HUANG Qing,QIAO Qilong,YANG Panpan,LI Yongtao,WANG Zeng,YANG Xia,MIAO Yuhe,ZHAO Jun.Construction and identification of recombinant fowl adenovirus 4 expressing the fiber gene of fowl adenovirus 8b[J].Chinese Journal of Veterinary Science,2022(2):230-236. |
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| Authors: | WANG Baiyu HUANG Qing QIAO Qilong YANG Panpan LI Yongtao WANG Zeng YANG Xia MIAO Yuhe ZHAO Jun |
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| Institution: | (College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China;Fujian Shengwei Biotech Co.,Ltd.,Nanping,Fujian 354100,China) |
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| Abstract: | A recombinant fowl adenovirus serotype 4(FAdV-4) expressing the Fiber gene of fowl adenovirus serotype 8 b(FAdV-8 b) was constructed in this study to establish a basis for the development of a novel vaccine that protect chickens against both hepatitis-hydropericardium syndrome(HHS) caused by FAdV-4 and inclusion body hepatitis(IBH) caused by FAdV-8 b.The full sequence of the fiber gene of FAdV-8 b epidemic strain was inserted into the 1 966 bp deletion area of FAdV-4 Chinese epidemic strain CH/HNJZ/2015 using a reverse genetic technique of FAdV-4.The rescued recombinant virus rHNJZ-Fiber/FAdV-8 b was identified by PCR,sequencing, Western blot, and indirect immunofluorescence assay.The results indicated that the Fiber gene was successfully inserted into the 1 966-bp deletion area and the Fiber protein was correctly expressed.The recombinant virus rHNJZ-Fiber/FAdV-8 b is genetically stable and has similar growth characteristics to the parent strain CH/HNJZ/2015.The recombinant virus rHNJZ-Fiber/FAdV-8 b will be a cost-effective candidate vaccine for the efficient control and prevention of HHS and IBH. |
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| Keywords: | fowl adenovirus 4 fowl adenovirus 8b Fiber protein hepatitis-hydropericardium syndrome inclusion body hepatitis novel vaccine |
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