| 猪源粪肠球菌和屎肠球菌多重PCR快速鉴定方法的建立 |
| |
| 引用本文： | 王亚宾,陈丽颖,胡慧,段志刚,崔保安.猪源粪肠球菌和屎肠球菌多重PCR快速鉴定方法的建立[J].中国兽医学报,2011,31(8):1123-1127. |
| |
| 作者姓名： | 王亚宾 陈丽颖 胡慧 段志刚 崔保安 |
| |
| 作者单位： | 河南农业大学牧医工程学院兽医微生物实验室,河南郑州,450002 |
| |
| 摘 要： | 粪肠球菌和屎肠球菌是引起猪感染发病的优势肠球菌种,以肠球菌的16S rRNA基因设计属特异性引物,利用SodA基因多态性设计种特异性引物,同时优化反应条件,建立了能同时测定猪源粪肠球菌和屎肠球菌多重PCR方法。通过特异性和敏感性分析,该方法能扩增出肠球菌属特异性片段及粪肠球菌和屎肠球菌种特异性片段;对粪肠球菌敏感性为0...
|
| 关 键 词： | 猪 肠球菌 多重PCR 16S rRNA Vitek-32 鉴定 |
Development of a multiplex PCR assay for detection of swine-originated E.faecalis and E.faecium isolates |
| |
| WANG Ya-bin,CHEN Li-ying,HU Hui,DUAN Zhi-gang,CUI Bao-an.Development of a multiplex PCR assay for detection of swine-originated E.faecalis and E.faecium isolates[J].Chinese Journal of Veterinary Science,2011,31(8):1123-1127. |
| |
| Authors: | WANG Ya-bin CHEN Li-ying HU Hui DUAN Zhi-gang CUI Bao-an |
| |
| Institution: | WANG Ya-bin,CHEN Li-ying,HU Hui,DUAN Zhi-gang,CUI Bao-an(The College of Animal Husbandy and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China) |
| |
| Abstract: | A method of multiplex PCR assay was developed for the quick detection of swine-originated Enterococcus faecalis and E.faecium isolates by using genus-primers based on the conserved regions 16S rRNA gene and species-specific primers on the SodA gene polymorphism.The specificity and sensitivity analysis showed that only Enterococci genus-and species-specific for E.faecalis and E.faecium fragments were obtained,with a minimal detectable dosage of 0.1 ng DNA for E.faecalis and that of 1 ng DNA for E.faecium.Gen... |
| |
| Keywords: | swine Enterococcus multiplex PCR 16SrRNA gene Vitek-32 identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
